Search

  • 주문 현황
  • 빠른주문
Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible, 25 mL - FAQs

Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible, 25 mL - FAQs

View additional product information for Pierce™ High Capacity Ni-IMAC MagBeads, EDTA compatible - FAQs (A50588, A50589, A50590, A50591)

7 product FAQs found

Can I automate purifications using Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible?

The KingFisher product line has proven to give high-throughput, high purity, and consistency across samples when performing automated purifications using Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

With Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible, which downstream assays are compatible with the eluted His-tagged protein?

Recovered protein can be run on SDS-PAGE and stained or analyzed by western blotting. Since the elution buffer contains 300 mM NaCl and 500 mM imidazole, recovered protein may need to be desalted or buffer-exchanged before proceeding to activity assays, mass spectrometry, or structural analyses.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

After regeneration of Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible, is there a specific buffer in which the beads should be stored?

The recommended storage buffer for the magnetic beads is 20% ethanol, 10 mM sodium acetate, pH 6.50. Please refer to the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0024861_PierceHighCapNiIMACBeads_UG.pdf) for details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

With Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible, how do I regenerate the beads?

Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible are alkali-stable. The beads can be exposed to NaOH, washed with water, neutralized with PBS, washed with water, and put back into storage buffer. Please refer to the product manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0024861_PierceHighCapNiIMACBeads_UG.pdf) for details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How many uses/regenerations are possible with Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible?

Up to 5 uses are possible with Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

With Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible, what is the size of the magnetic beads?

With Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible, the size of the magnetic beads is 30 micro;m.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the binding capacity of Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible?

The binding capacity of Pierce High Capacity Ni-IMAC MagBeads, EDTA compatible is up to 80 mg/mL.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.