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Phospho-PKR (Thr446) Polyclonal Antibody

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Phospho-PKR (Thr446) Polyclonal Antibody

Product Details

BS-3335R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:300
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1:500-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic phosphopeptide derived from human PKR around the phosphorylation site of Thr446.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: double stranded RNA activated protein kinase; dsRNA-activated kinase; EC 2.7.11.1; eIF-2 alpha; eIF-2A protein kinase 2; eIF2a Kinase; eukaryotic translation initiation factor 2 alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; PRKR; protein kinase R; Protein kinase RNA-activated; protein kinase RNA-regulated; protein kinase, interferon inducible double stranded RNA dependent; Protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Serine/threonine-protein kinase TIK; T-cell viral integration site; Tyrosine-protein kinase EIF2AK2; unnamed protein product

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Gene Aliases: 2310047A08Rik; 4732414G15Rik; AI467567; AI747578; DYT33; EIF2AK1; EIF2AK2; LEUDEN; PKR; PPP1R83; PRKR; Tik

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UniProt ID: (Human) P19525, (Mouse) Q03963, (Rat) Q63184

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Entrez Gene ID: (Human) 5610, (Mouse) 19106, (Rat) 54287

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Function(s)
nucleotide binding RNA binding double-stranded RNA binding protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding kinase activity transferase activity protein phosphatase regulator activity identical protein binding protein serine kinase activity transferase activity, transferring phosphorus-containing groups poly(A) RNA binding non-receptor serine/threonine protein kinase
Process(es)
positive regulation of cytokine production immune system process translation translational initiation regulation of translational initiation protein phosphorylation negative regulation of cell proliferation response to virus negative regulation of translation endoplasmic reticulum unfolded protein response positive regulation of chemokine production positive regulation of stress-activated MAPK cascade negative regulation of osteoblast proliferation cellular response to amino acid starvation response to interferon-alpha negative regulation of apoptotic process positive regulation of MAPK cascade negative regulation of viral genome replication innate immune response protein autophosphorylation positive regulation of NF-kappaB transcription factor activity defense response to virus antiviral innate immune response regulation of NLRP3 inflammasome complex assembly positive regulation of NIK/NF-kappaB signaling regulation of hematopoietic progenitor cell differentiation regulation of hematopoietic stem cell proliferation regulation of hematopoietic stem cell differentiation activation of MAPKK activity transcription, DNA-templated regulation of transcription, DNA-templated response to toxic substance phosphorylation response to lipopolysaccharide response to vitamin E positive regulation of apoptotic process response to mechanical stimulus response to organic substance peptidyl-tyrosine phosphorylation response to exogenous dsRNA
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