Invitrogen™

Novex™ pH 3-10 IEF Protein Gels, 1.0 mm, 10-well

Catalog number:  EC6655BOX

Related applications:

Protein Electrophoresis & Western Blotting

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Description

Novex® pH 3–10 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.

IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours.

Formulation
Novex® IEF gels are 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes. They do not contain denaturing reagents.

Recommended buffers
IEF is a sensitive technique which is affected by many factors. Our optimized, premixed IEF buffers reduce variability and help you obtain consistent results.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Mode of Separation: pI (Charge)
Product Line: Novex™
Sample Loading Volume: 25 µL
Shipping Condition: Wet Ice
System Type: Novex®
Thickness: 1.0 mm
Well Design: 1D Well
Length (Metric): 13.5 cm
Length (Metric) Gel: 8 cm
Width (Metric): 8 cm
Width (Metric) Gel: 8 cm
Product Type: Homogeneous Page
Gel Size: Mini
Separation Range: pH 3 to 10
Gel Percentage: 5%
Gel Type: Ampholyte
Separation Type: Isoelectric Focusing
Gel Thickness: 1 mm
For Use With (Equipment): Mini Gel Tank
Separation of: Protein
Wells: 10-well
Quantity: 5 Gels

Contents & storage

One box contains 5 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life up to 6 months.