Matrix architecture dictates three-dimensional migration modes of human macrophages: differential involvement of proteases and podosome-like structures.
AuthorsVan Goethem E, Poincloux R, Gauffre F, Maridonneau-Parini I, Le Cabec V,
JournalJ Immunol
PubMed ID20018633
'Tissue infiltration of macrophages, although critical for innate immunity, is also involved in pathologies, such as chronic inflammation and cancer. In vivo, macrophages migrate mostly in a constrained three-dimensional (3D) environment. However, in vitro studies, mainly focused on two dimensions, do not provide meaningful clues about the mechanisms involved in ... More
Studies of fibronectin matrices in living cells with fluoresceinated gelatin.
AuthorsHsieh P, Segal R, Chen LB
JournalJ Cell Biol
PubMed ID6998987
'We have used fluorescein isosthiocyanate-conjugated gelatin (FITC-gelatin) (1 mg/ml) to localize cell surface fibronectin in unfixed live cells in cultures. FITC-gelatin stains the fibronectin matrix on primary cultures of rat and chick embryo fibroblasts as well as untransformed, established cell lines. In live cultured cells, fibronectin in many areas of ... More
TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent.
Toll-like receptor (TLR) signaling induces a rapid reorganization of the actin cytoskeleton in cultured mouse dendritic cells (DC), leading to enhanced antigen endocytosis and a concomitant loss of filamentous actin-rich podosomes. We show that as podosomes are lost, TLR signaling induces prominent focal contacts and a transient reduction in DC ... More
Fluid-phase interaction between human plasma fibronectin and gelatin determined by fluorescence polarization assay.
AuthorsForastieri H, Ingham KC
JournalArch Biochem Biophys
PubMed ID6364982
Previous studies of the binding properties of fibronectin (Fn) have utilized methods whereby one or the other macromolecule was immobilized on a solid phase. In order to examine the interaction between human plasma Fn and gelatin in solution, the latter was labeled with fluorescein isothiocyanate (FITC) whose fluorescence polarization (P) ... More
Pathophysiology of premature skin aging induced by ultraviolet light.
AuthorsFisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ
JournalN Engl J Med
PubMed ID9358139
BACKGROUND: Long-term exposure to ultraviolet irradiation from sunlight causes premature skin aging (photoaging), characterized in part by wrinkles, altered pigmentation, and loss of skin tone. Photoaged skin displays prominent alterations in the collagenous extracellular matrix of connective tissue. We investigated the role of matrix-degrading metalloproteinases, a family of proteolytic enzymes, ... More
Susceptibility to tuberculosis is associated with variants in the ASAP1 gene encoding a regulator of dendritic cell migration.
Authors
JournalNat Genet
PubMed ID25774636
Treatment with DNases rescues hidden neutrophil elastase from aggregated NETs.
Authors
JournalJ Leukoc Biol
PubMed ID31478257
mTORC2-mediated PDHE1α nuclear translocation links EBV-LMP1 reprogrammed glucose metabolism to cancer metastasis in nasopharyngeal carcinoma.
Authors
JournalOncogene
PubMed ID30745576
Identification of a CIP4 PKA phosphorylation site involved in the regulation of cancer cell invasiveness and metastasis.
Authors
JournalCancer Lett
PubMed ID31319138
Inhibition of SHIP2 activity inhibits cell migration and could prevent metastasis in breast cancer cells.
Authors
JournalJ Cell Sci
PubMed ID30012834
Dynamin-2 Regulates Postsynaptic Cytoskeleton Organization and Neuromuscular Junction Development.
Authors
JournalCell Rep
PubMed ID33113375
An iPSC-derived vascular model of Marfan syndrome identifies key mediators of smooth muscle cell death.
Authors
JournalNat Genet
PubMed ID27893734
Magnetically attachable stencils and the non-destructive analysis of the contribution made by the underlying matrix to cell migration.
Authors
JournalBiomaterials
PubMed ID22940214
A free-form patterning method enabling endothelialization under dynamic flow.
Authors
JournalBiomaterials
PubMed ID33895492
Site-directed MT1-MMP trafficking and surface insertion regulate AChR clustering and remodeling at developing NMJs.