Informational:Inquire about OEM or Commercial Supply version of this product here.
Thermo Scientific™

Rapid DNA Ligation Kit

Catalog number: K1423
Thermo Scientific™

Rapid DNA Ligation Kit

Catalog number: K1423
Catalog Number
K1423
Unit Size
150 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
50 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K1423150 reactions
-
K142250 reactions
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.

Highlights

• Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
• Convenient—reaction mixture can be used directly for bacterial transformation

Applications

• Routine cloning experiments
• Blunt-end cloning
• Library construction
• TA cloning

Includes

T4 DNA Ligase
• 5X Rapid Ligation Buffer
Water, nuclease-free
• Detailed Protocol

Note

Prior to electroporation, it is necessary to inactivate T4 DNA ligase by chloroform extraction or spin column purification, e.g. with Thermo Scientific GeneJET PCR Purification Kit (K0701).

Related Products
Rapid DNA Ligation Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Sample Type
DNA
Enzyme
DNA Ligase
For Use With (Application)
Nucleic Acid Gel Electrophoresis
Product Type
Rapid DNA Ligation Kit
Quantity
150 Reactions

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text