Search
Invitrogen
ABRA1 Monoclonal Antibody (ABRA1-01)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
ABRA1 Antibody (MA1-10045) in WB
Western blotting detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells. Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01) Monoclonal antibody (Product # MA1-10045). Lane B: Immunostaining with anti-EGFP (polyclonal). Lane C: Immunostaining with anti-alpha tubulin (clone TU-01).
Product Details
MA1-10045
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
ABRA1-01
Immunogen
Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes human ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.
Target Information
CCDC98, also known as Abraxas 1, was identified through protein binding studies using the breast and ovarian predisposition protein BRCA1 as the binding target. CCDC98 recruits RAP80, a ubiquitin-binding protein, to BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both CCDC98 and RAP80 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either CCDC98 or RAP80 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that CCDC98 and RAP80 control only part of the DNA damage response role of BRCA1. At least two isoforms of CCDC98 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: abraxas protein; BRCA1 associated A-complex protein; BRCA1-A complex subunit Abraxas 1; coiled-coil domain containing 98; Coiled-coil domain-containing protein 98; family with sequence similarity 175 member A; FLJ11520; FLJ12642; FLJ13614; Protein FAM175A; unnamed protein product
Gene Aliases: ABRA1; ABRAXAS1; CCDC98; FAM175A; UNQ496/PRO1013
UniProt ID: (Human) Q6UWZ7
Entrez Gene ID: (Human) 84142
DNA repair
regulation of DNA repair
double-strand break repair
chromatin organization
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
attachment of spindle microtubules to kinetochore
response to ionizing radiation
mitotic G2/M transition checkpoint
positive regulation of DNA repair
mitotic spindle assembly
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support