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Invitrogen
Prohibitin Monoclonal Antibody (II-14-10), Biotin
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FIGURE: 1 / 5
Prohibitin Antibody (MA5-12855) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg of lysate) of Caco-2 (Lane 1), Caco-2 treated with H2O2 (100uM for 1 hr) (Lane2), Neuro-2a (Lane 3), Neuro-2a treated with H2O2 (100uM for 1 hr) (Lane 4), COS-7 (Lane 5), COS-7 treated with H2O2 (100uM for 1 hr) (Lane 6) and HepG2 (Lane 7). The blots were probed with anti-Prohibitin Mouse Monoclonal Antibody, Biotin (Product # MA5-12855, 1 µg/mL) and detected by chemiluminescence. A 30 kDa band corresponding to Prohibitin was observed across the cell lines tested. Kno... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-12855
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Published species
Avian,
Human,
Mouse,
Rat,
Virus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
II-14-10
Immunogen
Purified recombinant rat prohibitin protein
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-12855 targets Prohibitin in IF, IHC (P), and WB applications and shows reactivity with Chicken, Human, mouse, Porcine, and Rat samples.
The MA5-12855 immunogen is purified recombinant rat prohibitin protein.
Target Information
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell receptor-associated protein 32; epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; gastric cancer susceptibility protein; OTTHUMP00000165765; OTTHUMP00000165766; OTTHUMP00000220103; OTTHUMP00000220170; OTTHUMP00000220171; PHB; prohibitin; unnamed protein product
Gene Aliases: BAP32; HEL-215; HEL-S-54e; PHB
UniProt ID: (Human) P35232, (Mouse) P67778, (Rat) P67779
Entrez Gene ID: (Chicken) 419980, (Pig) 100524707, (Human) 5245, (Mouse) 18673, (Rat) 25344
negative regulation of transcription from RNA polymerase II promoter
ovarian follicle development
ovarian follicle atresia
positive regulation of immunoglobulin production
regulation of transcription, DNA-templated
mitochondrion organization
negative regulation of cell proliferation
response to xenobiotic stimulus
positive regulation of gene expression
negative regulation of gene expression
negative regulation of cell growth
organ regeneration
positive regulation of interleukin-17 production
response to cytokine
response to immobilization stress
RIG-I signaling pathway
regulation of gene expression, epigenetic
B cell activation
negative regulation of protein catabolic process
negative regulation of apoptotic process
response to peptide hormone
positive regulation of neuron apoptotic process
modulation by host of viral RNA genome replication
response to ethanol
positive regulation of G-protein coupled receptor protein signaling pathway
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of complement activation
viral entry into host cell
positive regulation of smooth muscle cell proliferation
protein stabilization
progesterone receptor signaling pathway
positive regulation of protein kinase B signaling
negative regulation of androgen receptor signaling pathway
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to interleukin-6
DNA biosynthetic process
T-helper 17 type immune response
antiviral innate immune response
positive regulation of NIK/NF-kappaB signaling
negative regulation of glucocorticoid receptor signaling pathway
osteoblast differentiation
signal transduction
regulation of apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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