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          • Primary Antibodies ›
          • NIT2 Antibodies

          Invitrogen

          NIT2 Monoclonal Antibody (OTI3D5)

          View all (17) NIT2 antibodies

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          Cite NIT2 Monoclonal Antibody (OTI3D5)

          Additional Information:
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          • Antibody Testing Data (6)
          NIT2 Antibody in Immunocytochemistry (ICC/IF)
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          NIT2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 6

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          NIT2 Antibody (MA5-25083) in ICC/IF

          Immunofluorescent analysis of NIT2 in COS7 cells. Cells were transfected with a plasmid overexpressing NIT2 and probed with a NIT2 monoclonal antibody (Product # MA5-25083). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          NIT2 Antibody in Immunocytochemistry (ICC/IF)
          NIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NIT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          NIT2 Antibody in Western Blot (WB)
          NIT2 Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          NIT2 Monoclonal Antibody (OTI3D5)

          Product Details

          MA5-25083

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -

          Flow Cytometry (Flow)

          1:100
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG2b

          Class

          Monoclonal

          Type

          Antibody

          Clone

          OTI3D5

          Immunogen

          Full length human recombinant protein of NIT2 produced in HEK293T cell
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.79 mg/mL

          Amount

          79 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 1% BSA, 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2724840

          Target Information

          Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: epididymis secretory sperm binding protein Li 8a; Nit protein 2; nitrilase homolog 2; unnamed protein product

          View more View less

          Gene Aliases: HEL-S-8a

          View more View less

          UniProt ID: (Human) Q9NQR4

          View more View less

          Entrez Gene ID: (Human) 56954

          View more View less

          Function(s)
          hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides omega-amidase activity 2-oxoglutaramate amidase activity
          Process(es)
          oxaloacetate metabolic process glutamine metabolic process L-methionine salvage
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Host server : magellan-search-green-5799cd5976-swbc5:80/100.66.77.38:80.
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          git-url: https://github.com/thermofisher/magellan-search
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