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Invitrogen

HSC70 Monoclonal Antibody (1F2-H5), APC

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HSC70 Monoclonal Antibody (1F2-H5), APC

Product Details

MA5-45189

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-

Functional Assay (Functional)

Assay-dependent
-

Proximity Ligation Assay (PLA) (PLA)

Assay-dependent
-

Immunomicroscopy (IM)

Assay-dependent
-

Antibody Microarray (Ab Microarray)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1F2-H5

Immunogen

Full length human HSC70
3D Epitope / Immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

200 µg

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931643

Product Specific Information

1 µg/mL of MA5-45189 was sufficient for detection of HSC70 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 73kDa. Does not cross react with HSP70.

Target Information

The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. HSC70 (also known as HSC71, HSC73, HSP73, p72, prp73) is expressed constitutively and is slightly heat-inducible. HSC70 binds to the exposed loop of clathrin light chains to promote uncoating and can also bind the cytoskeleton which may facilitate cytoskeletal rearrangements. HSC70 has been shown to stimulate lysosomal degradation of intracellular proteins and to retard both aggregation and folding of mitochondrial precursor proteins in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AAG2; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; FAM10A1; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kD protein 8; heat shock 70kDa protein 8; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein cognate 70; Heat shock protein family A member 8; HIP; LAP; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; MGC131511; MGC29929; N-myristoyltransferase inhibitor protein 71; Protein FAM10A1; SNC6; unnamed protein product

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Gene Aliases: 2410008N15Rik; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; Hsc73; HSP71; HSP73; HSPA10; HSPA8; LAP-1; LAP1; NIP71

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UniProt ID: (Human) P11142, (Rat) P63018, (Mouse) P63017

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Entrez Gene ID: (Human) 3312, (Rat) 24468, (Mouse) 15481

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Function(s)
nucleotide binding G-protein coupled receptor binding RNA binding protein binding ATP binding hydrolase activity ATPase activity enzyme binding MHC class II protein complex binding protein binding, bridging heat shock protein binding ubiquitin protein ligase binding protein binding involved in protein folding cadherin binding receptor agonist activity unfolded protein binding chaperone binding C3HC4-type RING finger domain binding ATP-dependent protein disaggregase activity ATP-dependent protein folding chaperone receptor binding ATPase activity, coupled ADP binding poly(A) RNA binding
Process(es)
transcription, DNA-templated mRNA processing protein folding RNA splicing protein refolding negative regulation of transcription, DNA-templated positive regulation of mRNA splicing, via spliceosome chaperone mediated protein folding requiring cofactor regulation of cell cycle chaperone-mediated protein folding clathrin coat disassembly negative regulation of fibril organization mRNA splicing, via spliceosome autophagy response to unfolded protein signal transduction cellular response to starvation negative regulation of signal transduction protein catabolic process positive regulation of cell migration regulation of protein stability cellular response to stress regulation of cytoplasmic pattern recognition receptor signaling pathway regulation of protein complex assembly ATP metabolic process negative regulation of cellular component organization membrane organization regulation of protein complex stability chaperone-mediated autophagy protein targeting to lysosome involved in chaperone-mediated autophagy cellular response to steroid hormone stimulus negative regulation of NLRP3 inflammasome complex assembly regulation of fibril organization regulation of protein import chaperone-mediated autophagy translocation complex disassembly regulation of transcription, DNA-templated
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