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Invitrogen
Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody (PSH01-81)
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FIGURE: 1 / 13
Aldolase A/B/C Antibody (MA5-56379) in ICC/IF
Immunofluorescence analysis of Aldolase A/B/C using U-87 MG cells. The cells were fixed 4% paraformaldehyde for 20 minutes, permeabilized with 0.1% Triton™ X-100 in PBS for 5 minutes, and blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were co-labeled with Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody (PSH01-81) (Product # MA5-56379, green) and beta tublin (r... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-56379
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH01-81
Immunogen
Synthetic peptide within human aldolase c aa 50-100.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Positive Control: U-87 MG cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, mouse pancreas tissue lysate, rat brain tissue lysate, rat pancreas tissue lysate, U-87 MG, mouse brain tissue, rat brain tissue.
Tissue Specificity: Group enriched (brain, retina).
Subcellular Location: cytoskeleton, cytosol, extracellular exosome, extracellular region.
Sequence Similarities: 100% Mouse/Rat.
Predicted band size: 110 kDa.
Target Information
Aldolases are important glycolytic cytosolic enzymes which catalyse the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate. There are three aldolase isozymes coded by three distinct genes in mammals, namely aldolases A, B, and C. Aldolase A is heavily expressed in muscle enzyme and aldolase B is heavily expressed in liver and is regarded as a liver specific enzyme. In the adult, aldolase C is the brain-specific isozyme expressed in astrocytes and a few classes of neurons, notably Purkinje cells. Appropriate antibodies to aldolase C are therefore useful to identify astrocytes in cell culture and sectioned material, and the enzyme may be over expressed in some forms of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALDA; ALDB; ALDC; ALDOA; ALDOC; aldolase 2; aldolase 3; aldolase A; aldolase A (EC 4.1.2.13); aldolase A (EC 4.1.3.13); aldolase A, fructose-bisphosphate; aldolase B; Aldolase B, fructose-biphosphate; aldolase B, fructose-bisphosphatase; aldolase B, fructose-bisphosphate; aldolase C, fructose-biphosphate; aldolase C, fructose-bisphosphate; brain-type aldolase; epididymis secretory sperm binding protein; epididymis secretory sperm binding protein Li 87p; fructoaldolase C; fructose 1,6-diphosphate aldolase A (EC 4.1.2.13); fructose-1,6-biphosphate triosephosphate lyase; fructose-1,6-bisphosphate triosephosphate-lyase; gluconeogenic enzyme; ALDOA_V1; gluconeogenic enzyme; ALDOA_V2; liver-type aldolase; lung cancer antigen NY-LU-1; muscle-type aldolase; scrapie responsive gene 2; scrapie-responsive protein 2; unnamed protein product; zebrin II
Gene Aliases: ALDA; ALDB; ALDC; ALDCAA; Aldo-1; Aldo-2; Aldo1; ALDO2; Aldo3; F16dip7; GSD12; HEL-S-87p; LIV10; RATALDCAA; RNALDOG5; Scrg2
UniProt ID: (Human) P04075, (Human) P05062, (Human) P09972, (Rat) P05065, (Mouse) P05064, (Mouse) Q91Y97, (Rat) P09117, (Mouse) P05063
Entrez Gene ID: (Human) 226, (Human) 229, (Human) 230, (Rat) 24189, (Mouse) 11674, (Rat) 24190, (Mouse) 230163, (Rat) 24191, (Mouse) 11676
RNA binding
actin binding
fructose-bisphosphate aldolase activity
protein binding
cytoskeletal protein binding
tubulin binding
identical protein binding
cadherin binding
fructose binding
ATPase binding
binding, bridging
fructose-1-phosphate aldolase activity
protease binding
phosphatidylcholine binding
macromolecular complex binding
response to hypoxia
fructose metabolic process
glycolytic process
ATP biosynthetic process
striated muscle contraction
binding of sperm to zona pellucida
regulation of cell shape
methylglyoxal biosynthetic process
fructose 1,6-bisphosphate metabolic process
response to lipopolysaccharide
response to nicotine
positive regulation of insulin secretion involved in cellular response to glucose stimulus
response to estrogen
muscle cell cellular homeostasis
protein homotetramerization
canonical glycolysis
gluconeogenesis
epithelial cell differentiation
organ regeneration
hexose metabolic process
positive regulation of cell migration
cellular response to glucose starvation
glycolytic process through fructose-6-phosphate
liver development
fructose catabolic process
response to xenobiotic stimulus
response to carbohydrate
response to fructose
response to zinc ion
cellular response to insulin stimulus
response to starvation
response to amino acid
response to peptide hormone
response to copper ion
response to glucocorticoid
response to cAMP
vacuolar proton-transporting V-type ATPase complex assembly
response to interleukin-6
negative regulation of pentose-phosphate shunt
glycolytic process through fructose-1-phosphate
actin filament organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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