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Invitrogen
Fibrillarin Recombinant Rabbit Monoclonal Antibody (6H5A8)
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FIGURE: 1 / 7
Fibrillarin Antibody (MA569753) in ICC/IF
Immunocytochemical analysis of Fibrillarin in NIH/3T3 cells. Samples were incubated with Fibrillarin recombinant monoclonal antibody (Product # MA569752) using a dilution of 1:200 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). The cells were counterstained with alpha-Tubulin Mouse mAb using dilution of 1:400. Followed by incubation with 488-... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA569753
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6H5A8
Immunogen
Synthetic peptide.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.77 mg/mL
Amount
15.4 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3., with 50% glycerol, 0.05% BSA
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13. 1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; fibrillarin; histone-glutamine methyltransferase; nucleolar protein 1; RNA, U3 small nucleolar interacting protein 1; U6 snRNA 2'-O-methyltransferase fibrillarin; unnamed protein product
Gene Aliases: FIB; FLRN; Nop1; RNU3IP1
UniProt ID: (Human) P22087, (Rat) P22509, (Mouse) P35550
Entrez Gene ID: (Human) 2091, (Rat) 292747, (Mouse) 14113
TFIID-class transcription factor binding
RNA binding
protein binding
methyltransferase activity
rRNA methyltransferase activity
protein-glutamine N-methyltransferase activity
ATPase binding
RNA 2'-O-methyltransferase activity
U6 snRNA 2'-O-ribose methyltransferase activity
histone-glutamine methyltransferase activity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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