The microvasculature of the hamster cheek pouch as a model for studying acute immune-complex-induced inflammatory reactions (with 1 color plate).
AuthorsBjörk J, Smedegård G
JournalInt Arch Allergy Appl Immunol
PubMed ID6232222
'The acute microvascular reactions induced by immune complexes were studied. Hamster cheek pouch preparations of ovalbumin (OA)-immunized animals were topically exposed to different doses of OA (0.1, 1, 10 or 100 micrograms/ml). Fluorescein-isothiocyanate-labelled dextran (FITC-dextran, molecular weight 150,000) was injected intravenously as a macromolecular tracer. The microvascular alterations observed upon ... More
Low molecular weight antigen arrays delete high affinity memory B cells without affecting specific T-cell help.
AuthorsReim JW, Symer DE, Watson DC, Dintzis RZ, Dintzis HM
JournalMol Immunol
PubMed ID9171897
'An ongoing, T-cell dependent, secondary antibody response to an epitope can be suppressed in vivo by low molecular weight, soluble polymers, bearing multiple copies of the same epitope. This study illustrates that such suppressive T-cell independent antigen arrays target the epitope-specific, high affinity, memory B cells for long-term functional elimination. ... More
Permeability of the primary decidual zone in the rat uterus: studies using fluorescein-labeled proteins and dextrans.
AuthorsParr MB, Parr EL
JournalBiol Reprod
PubMed ID2420379
'The primary decidual zone (PDZ) is a transitory avascular region of transformed fibroblasts surrounding the luminal epithelium at the implantation site. Since this zone may restrict the passage of immunoglobulins, cells, nutrients, and other substances from maternal blood to the epithelium and embryo from Days 6 to 8 of pregnancy, ... More
Hepatic sinusoidal endothelium heterogeneity with respect to mannose receptor activity is interleukin-1 dependent.
AuthorsAsumendi A, Alvarez A, Martinez I, Smedsrød B, Vidal-Vanaclocha F
JournalHepatology
PubMed ID8675173
'Using fluorescein isothiocyanate-conjugated ovalbumin (OVA-FITC), 125I-mannan, or 125I-invertase as specific ligands for the mannose receptor, we have quantified its activity in mouse and rat hepatic sinusoidal endothelium (HSE), under both basal conditions and after lipopolysaccharide (LPS) or human recombinant interleukin-1beta (IL-1beta) stimulations. Mouse treatment for 4 hours with 5 microg/kg ... More
Preparation of fluorescent derivatives of lipases and their use in fluorescence energy transfer studies in hydrocarbon/water interfaces.
AuthorsRoberts GA, Tombs MP
JournalBiochim Biophys Acta
PubMed ID3113482
'Fluorescein isothiocyanate reacted with a chromobacter and pseudomonad lipase to yield mono-substituted, fully active, enzymes. With the carbocyanine dye 1,1''-dioctadecyl-3,3,3'',3''-tetramethylindocarbocyanine perchlorate (DiI) in the non-aqueous phase, fluorescence energy transfer was used to follow the lipase and similarly labelled model proteins in and out of the interface in heptane, and heptane/di-O-palmitoyl-rac-glycerol ... More
Impaired dendritic cell maturation and increased T(H)2 responses in PIR-B(-/-) mice.
AuthorsUjike A, Takeda K, Nakamura A, Ebihara S, Akiyama K, Takai T
JournalNat Immunol
PubMed ID12021780
'Mice deficient for paired immunoglobulin (Ig)-like receptor B (PIR-B) show defective regulation of receptor-mediated activation in antigen-presenting cells. Older PIR-B(-/-) mice had an increased number of peritoneal B1 cells. Splenic PIR-B(-/-) B2 cells were constitutively activated and proliferated much more than those from wild-type mice upon B cell receptor ligation. ... More
Distribution of actin in spreading macrophages: a comparative study on living and fixed cells.
AuthorsAmato PA, Unanue ER, Taylor DL
JournalJ Cell Biol
PubMed ID6339523
Microfluorometric immunoassays with antigens bound to sepharose beads.
AuthorsHaaijman JJ, Bloemmen FJ, Ham CM
JournalAnn N Y Acad Sci
PubMed ID1101767
'Sepharose beads, to which protein had been coupled, were used in the development of two types of microfluorometric immunoassays. The first is an inhibition assay: a fixed amount of antiserum was absorbed with different amounts of antigen. This was accomplished in both direct and indirect incubation schemes. The system has ... More
Effects of cytokines on mycobacterial phagosome maturation.
AuthorsVia LE, Fratti RA, McFalone M, Pagan-Ramos E, Deretic D, Deretic V
JournalJ Cell Sci
PubMed ID9490634
'One of the major mechanisms permitting intracellular pathogens to parasitize macrophages is their ability to alter maturation of the phagosome or affect its physical integrity. These processes are opposed by the host innate and adaptive immune defenses, and in many instances mononuclear phagocytes can be stimulated with appropriate cytokines to ... More
Use of antibodies against the label in non-separation non-isotopic immunoassay: 'indirect quenching' fluoroimmunoassay of proteins.
AuthorsNargessi RD, Landon J, Smith DS
JournalJ Immunol Methods
PubMed ID379224
'Antibodies against the label are introduced as a potentially useful reagent in nonisotopic immunoassay. They may permit end point determination without the need for a separation step, provided (i) that steric hindrance selectively prevents their binding to the antibody-bound fraction of the labelled antigen in an immunoassay mixture, and (ii) ... More
Delivery of macromolecules into cytosol using liposomes containing hemolysin from Listeria monocytogenes.
AuthorsLee KD, Oh YK, Portnoy DA, Swanson JA
JournalJ Biol Chem
PubMed ID8631734
'The cytosolic space of cells is an important but relatively inaccessible target for the delivery of therapeutic macromolecules. Here we describe the efficient delivery of macromolecules into the cytosolic space of macrophages from liposomes that contain listeriolysin O (LLO), the hemolytic protein of Listeria monocytogenes that normally mediates bacterial passage ... More
pH changes in pinosomes and phagosomes in the ameba, Chaos carolinensis.
AuthorsHeiple JM, Taylor DL
JournalJ Cell Biol
PubMed ID7119011
'Changes in pH are measured in pinosomes and phagosomes of single specimens of the giant, free-living ameba, Chaos carolinensis. Measurements of pH are made microfluorometrically, as previously described (Heiple and Taylor. 1980. J. Cell Biol. 86:885-890.) by quantitation of fluorescence intensity ratios (Ex489nm,/Ex452nm, Em520-560nm from ingested fluorescein thiocarbamyl (FTC)-ovalbumin. After ... More
Fluorescent analog cytochemistry of contractile proteins.
AuthorsWang YL, Heiple JM, Taylor DL
JournalMethods Cell Biol
PubMed ID7202103
A stable, high capacity, F-actin affinity column.
AuthorsLuna EJ, Wang YL, Voss EW, Branton D, Taylor DL
JournalJ Biol Chem
PubMed ID7130194
A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG that is covalently bound to Sepharose 4B. When stabilized with phalloidin, the actin remains associated with the Sepharose beads during repeated washes, activates the ATPase activity of myosin subfragment 1, and specifically binds 125I-heavy meromyosin ... More
Rheology of G-actin solutions.
AuthorsZaner KS, King RG, Newman J, Schick KL, Furukawa R, Ware BR
JournalJ Biol Chem
PubMed ID3366773
Recent reports that dilute solutions of G-actin form viscoelastic gels have been investigated using several experimental variations of three distinct physical techniques in independent laboratories. Direct measurement of storage and loss moduli were made using two rheometers of different design. Measurements of the diffusion of G-actin and of tracer particles ... More
Induction and down-regulation of conjunctival type-I hypersensitivity reactions in guinea pigs sensitized topically with fluoresceinyl ovalbumin.
AuthorsKhatami M, Donnelly JJ, Rockey JH
JournalOphthalmic Res
PubMed ID4011127
Type-I hypersensitivity reactions were induced in guinea pigs by repeated topical/conjunctival application of fluoresceinyl ovalbumin (FL-OA). The ocular reactivity in early responding animals was maximal between 16 and 25 days and decreased exponentially thereafter. Desensitized eyes responded minimally to compound 48/80 but maximally to histamine. Unilateral sensitization and challenge with ... More
Laser excitation of fluorescent-labeled polypeptides in polyacrylamide gels.
AuthorsDuffy VE, Stockwell PA, Warrington DM, Monk BC
JournalAnal Biochem
PubMed ID2505638
A laser beam at 488 nm, converted into a fan of light by a surface-coated mirror oscillated in response to a triangular wave, was inserted into the base of a polyacrylamide gel. The laser light was trapped by internal reflection and gave uniform illumination throughout the entire gel slab. Photography ... More
Fc receptor-directed phagocytic stimuli induce transient actin assembly at an early stage of phagocytosis in neutrophil leukocytes.
AuthorsSheterline P, Rickard JE, Richards RC
JournalEur J Cell Biol
PubMed ID6734633
The phagocytic pathway for the uptake of multivalent Fc complexes by neutrophil leukocytes can be partially dissected by using a pulse-chase protocol at different chase temperatures. Utilising a deoxyribonuclease I inhibition assay, we show that an early response to recognition of phagocytic substrate by neutrophils is a rapid (less than ... More
Direct cytotoxicity mediated by allogeneic and isogeneic pig lymphocytes modified with fluorescein isothiocyanate.
AuthorsMorilla A, Licence ST, Binns RM
JournalRev Latinoam Microbiol
PubMed ID1670253
Cell mediated cytotoxicity (CMC) is restricted by the major histocompatibility complex (MHC) in some animal models but not in others. In order to determine if there was MHC restriction in a CMC experimental model, mononuclear cells from blood of two lines of pigs, (1/1) and (6/6) which are homogeneous to ... More
On-chip coupling of isoelectric focusing and free solution electrophoresis for multidimensional separations.
AuthorsHerr AE, Molho JI, Drouvalakis KA, Mikkelsen JC, Utz PJ, Santiago JG, Kenny TW
JournalAnal Chem
PubMed ID12641239
We have developed an acrylic microfluidic device that sequentially couples liquid-phase isoelectric focusing (IEF) and free solution capillary electrophoresis (CE). Rapid separation (<1 min) and preconcentration (73x) of species were achieved in the initial IEF dimension. Using full-field fluorescence imaging, we observed nondispersive mobilization velocities on the order of 20 ... More
Distribution of fluorescently labeled actin in living sea urchin eggs during early development.
AuthorsWang YL, Taylor DL
JournalJ Cell Biol
PubMed ID457779
Rabbit skeletal muscle actin was labeled with 5-iodoacetamidofluorescein (5-IAF) and purified by gel filtration, ion-exchange chromatography, and polymerization-depolymerization. The resultant fluorescent conjugates retained full biochemical activities. The labeled actin was incorporated into unfertilized eggs of Lytechinus pictus by direct microinjection and the distribution of fluorescence was investigated after fertilization through ... More
Different cytokines regulate antigen uptake and presentation of a precursor dendritic cell line.
AuthorsLutz MB, Assmann CU, Girolomoni G, Ricciardi-Castagnoli P
JournalEur J Immunol
PubMed ID8605925
Langerhans cells (LC) and dendritic cells (DC) need to be activated in order to perform their antigen-presenting function. In this study, we explored the influence of cytokines on the uptake and presentation of protein antigens by the retrovirally immortalized myeloid cell line FSDC. This cell line was generated from mouse ... More
Differential production of chemokines and their role in neutrophil infiltration in rat allergic inflammation.
AuthorsNakagawa H, Ando Y, Takano K, Sunada Y
JournalInt Arch Allergy Immunol
PubMed ID9482702
BACKGROUND: Recently we demonstrated that activated rat macrophages produced neutrophil chemotactic factors (chemokines) including cytokine-induced neutrophil chemoattractant (CINC)-1, CINC-2alpha, CINC-2beta, CINC-3/rat macrophage inflammatory protein (MIP)-2 and rat MIP-1alpha (rMIP-1alpha). METHODS: In the present study, by using an enzyme-linked immunosorbent assay specific for each chemokine, we determined the levels of the ... More
Tubulin dynamics in cultured mammalian cells.
AuthorsSaxton WM, Stemple DL, Leslie RJ, Salmon ED, Zavortink M, McIntosh JR
JournalJ Cell Biol
PubMed ID6501419
Bovine neurotubulin has been labeled with dichlorotriazinyl-aminofluorescein (DTAF-tubulin) and microinjected into cultured mammalian cells strains PTK1 and BSC. The fibrous, fluorescence patterns that developed in the microinjected cells were almost indistinguishable from the pattern of microtubules seen in the same cells by indirect immunofluorescence. DTAF-tubulin participated in the formation of ... More
Transfer of antigen between dendritic cells in the stimulation of primary T cell proliferation.
AuthorsKnight SC, Iqball S, Roberts MS, Macatonia S, Bedford PA
JournalEur J Immunol
PubMed ID9603470
Primary proliferative T cell responses require stimulation with antigen-pulsed dendritic cells (Ag-DC). Here we show that for optimal stimulation, dendritic cells (DC) not exposed directly to antigen are also required. Ag-DC added to DC-depleted T cells caused negligible primary stimulation; adding back DC resulted in stimulation. These effects were seen ... More
Diffusion and partitioning of proteins in charged agarose gels.
AuthorsJohnson EM, Berk DA, Jain RK, Deen WM
JournalBiophys J
PubMed ID7787041
The effects of electrostatic interactions on the diffusion and equilibrium partitioning of fluorescein-labeled proteins in charged gels were examined using fluorescence recovery after photobleaching and gel chromatography, respectively. Measurements were made with BSA, ovalbumin, and lactalbumin in SP-Sepharose (6% sulfated agarose), in phosphate buffers at pH 7 and ionic strengths ... More
Microspectrofluorometry by digital image processing: measurement of cytoplasmic pH.
AuthorsTanasugarn L, McNeil P, Reynolds GT, Taylor DL
JournalJ Cell Biol
PubMed ID6198329
An interface of our microspectrofluorometer with an image processing system performs microspectrofluorometric measurements in living cells by digital image processing. Fluorescence spectroscopic parameters can be measured by digital image processing directly from microscopic images of cells, and are automatically normalized for pathlength and accessible volume. Thus, an accurate cytoplasmic "map" ... More
The actin-binding protein comitin (p24) is a component of the Golgi apparatus.
AuthorsWeiner OH, Murphy J, Griffiths G, Schleicher M, Noegel AA
JournalJ Cell Biol
PubMed ID8408201
Comitin (p24) was first identified in Dictyostelium discoideum as a membrane-associated protein which binds in gel overlay assays to G and F actin. To analyze its actin-binding properties we used purified, bacterially expressed comitin and found that it binds to F actin in spin down experiments and increases the viscosity ... More
Intracellular pH in single motile cells.
AuthorsHeiple JM, Taylor DL
JournalJ Cell Biol
PubMed ID7410485
Cytoplasmic pH in single living specimens of Chaos carolinensis is determined microfluorometrically by measuring the ratio of fluorescence intensity of microinjected fluorescein-thiocarbamyl (FTC)-ovalbumin at two different excitation wavelengths. The probe is evenly distributed throughout, and confined to, the cytoplasm, and the fluorescence intensity ratio depends only upon pH. It is ... More
Polyethylene glycol-modified GM-CSF expands CD11b(high)CD11c(high) but notCD11b(low)CD11c(high) murine dendritic cells in vivo: a comparative analysis with Flt3 ligand.
AuthorsDaro E, Pulendran B, Brasel K, Teepe M, Pettit D, Lynch DH, Vremec D, Robb L, Shortman K, McKenna HJ, Maliszewski CR, Maraskovsky E
JournalJ Immunol
PubMed ID10861034
Dendritic cells (DC) are potent APCs that can be characterized in the murine spleen as CD11b(high)CD11c(high) or CD11b(low)CD11c(high). Daily injection of mice of Flt3 ligand (FL) into mice transiently expands both subsets of DC in vivo, but the effect of administration of GM-CSF on the expansion of DC in vivo ... More
Intracellular routes and selective retention of antigens in mildly acidic cathepsin D/lysosome-associated membrane protein-1/MHC class II-positive vesicles in immature dendritic cells.
Immature dendritic cells (DC) use both macropinocytosis and mannose receptor-mediated endocytosis to internalize soluble Ags efficiently. These Ags are ultimately presented to T cells after DC maturation and migration into the lymph nodes. We have previously described the immortalized myeloid cell line FSDC as displaying the characteristics of early DC ... More
Endocytic vesicles from renal papilla which retrieve the vasopressin-sensitive water channel do not contain a functional H+ ATPase.
AuthorsLencer WI, Verkman AS, Arnaout MA, Ausiello DA, Brown D
JournalJ Cell Biol
PubMed ID1696262
The water permeability of the kidney collecting duct epithelium is regulated by vasopressin (VP)-induced recycling of water channels between an intracellular vesicular compartment and the plasma membrane of principal cells. To test whether the water channels pass through an acidic endosomal compartment during the endocytic portion of this pathway, we ... More
Analysis of the insulin receptor by anti-receptor antibodies and flow cytometry.
AuthorsMaron R, Jackson RA, Jacobs S, Eisenbarth G, Kahn CR
JournalProc Natl Acad Sci U S A
PubMed ID6390439
We characterized insulin receptors on a human lymphoblastoid cell line (IM-9) and studied their regulation using anti-receptor antibodies and fluorescence flow cytometry. The fluorescence intensity distribution of insulin receptors on cells was determined by incubating the cells with one of three different anti-receptor antisera (human serum B-9 containing polyclonal autoantibodies, ... More
Sex-related differences in the handling of fluorescent ovalbumin by the proximal tubule of the rat kidney.
AuthorsAsan E, Kugler P, Schiebler TH
JournalHistochemistry
PubMed ID3721912
Sex-dependent protein handling in the rat renal tubular system was studied both qualitatively and quantitatively using the method of direct fluorescent protein tracing. The protein tracer, fluorescent ovalbumin, was synthesized by conjugating hen ovalbumin with fluorescein isothiocyanate (FITC), and the fluorescence characteristics of fluoresceinthiocarbamyl (FTC)-ovalbumin conjugates with different degrees of ... More
Prediction of permeability-surface area product data by continuous-distribution pore models.
AuthorsMcNamee JE, Wolf MB
JournalMicrocirculation
PubMed ID9866118
OBJECTIVE: To investigate the ability of continuous-distribution pore models to accurately predict permeability-surface area product (PS) experimental data in skeletal muscle. METHODS: Models having a water-only (WO) pathway and continuous distributions of microvascular transport-pathway sizes were fit to solute reflection-coefficient (sigma) experimental data (approximately 0.5-16 nm Stokes radii) obtained from ... More
Identification of microvascular transport pathways in skeletal muscle.
AuthorsWolf MB
JournalAm J Physiol
PubMed ID8048604
The solvent-drag reflection coefficient (sigma f) was measured from plasma disappearance (integral-mass balance method) for native albumin and four fluorescent solutes of radii from 2 to 16 nm in the isolated, plasma-perfused cat hindlimb preparation. The data for the smallest solutes were measured > 2 h after tracer addition and ... More
Ssu72 regulates alveolar macrophage development and allergic airway inflammation by fine-tuning of GM-CSF receptor signaling.
Authors
JournalJ Allergy Clin Immunol
PubMed ID32910932
Single-Cell Proteomics Reveals the Defined Heterogeneity of Resident Macrophages in White Adipose Tissue.
Authors
JournalFront Immunol
PubMed ID34381461
Solute transport in growth plate cartilage: in vitro and in vivo.
Authors
JournalBiophys J
PubMed ID17496046
Neonatal Fc receptor induces intravenous immunoglobulin growth suppression in Langerhans cell histiocytosis.
Authors
JournalPathol Int
PubMed ID33497038
Optimization of methods to study pulmonary dendritic cell migration reveals distinct capacities of DC subsets to acquire soluble versus particulate antigen.
Authors
JournalJ Immunol Methods
PubMed ID18662693
Generation and characterization of regulatory dendritic cells derived from murine induced pluripotent stem cells.
Authors
JournalSci Rep
PubMed ID24496181
FcγR engagement reprograms neutrophils into antigen cross-presenting cells that elicit acquired anti-tumor immunity.
Authors
JournalNat Commun
PubMed ID34373452
Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms.
Authors
JournalNat Commun
PubMed ID34876561
Carbon monoxide impairs mitochondria-dependent endosomal maturation and antigen presentation in dendritic cells.
Authors
JournalEur J Immunol
PubMed ID26461179
Bone marrow endothelial cells sustain a tumor-specific CD8+ T cell subset with suppressive function in myeloma patients.