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Thermo Scientific™

MAP2K1 (MEK1) [S218D] [S222D] Recombinant Human Protein

Catalog number:  P3099

Related applications:

Protein Assays and Analysis

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Description

MAP2K1 (MEK1) kinse is a dual-specific protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation. MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx. This mutant has been activated by subsituting serines 218 and 222 with aspartic acid to mimic the phosphorylated state.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NP_002746, S218D, S222D
Assay Entry: Biochemical binding/kinetic
Kinase Group: Ser⁄Thr Kinases: STE Group
Mass: 10 µg
Shipping Condition: Dry Ice
Tag Position: N-Terminal
Target Entry: MAP2K1 S218D S222D
Target Gene: Mitogen-activated protein kinase kinase 1
Expression System: Baculovirus
Protein Family: Kinases & Inhibitors
Protein Form: Full Length, Recombinant
Protein Subtype: Serine⁄Threonine Kinases
Research Category: Signal Transduction
Species: Human
Validated Application: Kinase Assay
Gene ID (Entrez): 5604
Molecular Weight: 49.2 kDa
Gene Alias: MAP2K1 (MEK1) S218D S222D
KinaseFamily: STE7 Kinase Family
Protein Tag: His Tag

Contents & storage

Storage Buffer: 50 mM Tris, pH 7.5, 150 mM NaCl, 0.05 mM EDTA, 0.05% NP-40, 1mM DTT, 50% glycerol
Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.