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Invitrogen
SPDYA Polyclonal Antibody
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FIGURE: 1 / 4
SPDYA Antibody (PA1-16959) in IHC (P)
Immunohistochemical analysis of SPDYA in immersion fixed paraffin-embedded sections of human testis. Samples were incubated in SPDYA polyclonal antibody (Product # PA1-16959) using a dilution of 1:300 for 1 hour at room temperature followed by the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized t... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA1-16959
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Sheep,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide made to a C-terminal region of human Speedy/Ringo (within residues 200-286).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.1 mg/mL
Amount
110 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/tris phosphate, pH 7-8
Contains
0.1% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-16959 is specific for the ~36 kDa Speedy/Ringo protein (isoform 2).
After a longer exposure, a 31 kDa band can be detected (isoform 1).
There is also a non-specific band detected at ~58 kDa.Suggested positive control: mouse testis lysate, mouse testis protein.
Target Information
Speedy A, also known as SPDYA, SPDY1, Ringo3 or SPY1, is a 313 amino acid protein that localizes to the nucleus and belongs to the speedy/ringo family. Expressed at high levels in testis and at lower levels in brain, kidney, heart, bone marrow, colon, lung, liver spleen and placenta, Speedy A functions to regulate the G1/S phase transition of the cell cycle, specifically by binding to and activating Cdc2 p34, Cdk2 and p27. Additionally, Speedy A mediates cell survival during DNA damage, suggesting that Speedy A plays a role in proper cell cycle progression throughout the life of the cell. Multiple isoforms of Speedy A exist due to alternative splicing events. The gene encoding Speedy A maps to human chromosome 2, which encodes over 1,400 genes and comprises nearly 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; hSpy/Ringo A; mSpy/Ringo A; protein expressed in male leptotene and zygotene spermatocytes 465; rapid inducer of G2/M progression in oocytes A; RINGO A; speedy A; speedy A1; speedy A2; speedy homolog 1; speedy homolog A; speedy-1; speedy/ringo A1; speedy/ringo A2; spermatogenesis related protein Gs4; spy1; Spy1; speedy/ringo A1; unnamed protein product
Gene Aliases: 4921517J08Rik; 4930548B21Rik; GS4; Lm23; MLZ-465; RINGO3; RINGOA; SPDY1; SPY1
UniProt ID: (Human) Q5MJ70, (Mouse) Q5IBH7, (Rat) Q8R496
Entrez Gene ID: (Human) 245711, (Sheep) 101123454, (Mouse) 70891, (Rat) 192209
G1/S transition of mitotic cell cycle
cellular response to DNA damage stimulus
male meiosis
spermatogenesis
positive regulation of cell proliferation
telomere capping
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of protein kinase activity
oogenesis
meiotic attachment of telomere to nuclear envelope
establishment of protein localization to telomere
positive regulation of gene expression
positive regulation of meiotic cell cycle process involved in oocyte maturation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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