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Antibody Testing Data (2)
Advanced Verification (1)

FIGURE: 1 / 3

CLOCK Antibody (PA3-16514) in WB

Western blot was performed using Anti-CLOCK Polyclonal Antibody (Product # PA3-16514) and a 95kDa band corresponding to CLOCK was observed across the cell lines and tissue extracts tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), MCF-7 (Lane 3), MCF-7 treated with Beta Estradiol (1uM for 24hrs) (Lane 4), T-47D (Lane 5), PC-3 (Lane 6), NIH/3T3 (Lane 7), tissue extracts of Mouse Testis (Lane 8), Mouse Skeletal muscle (Lane 9), Rat Skeletal muscle (Lane 10) and Mouse Thymus (Lane 11) were electrop... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

CLOCK Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Anti-CLOCK Polyclonal Antibody (Product # PA3-16514), shows increased expression of CLOCK in MCF-7 cell line upon Beta Estradiol treatment. {TM}

Product Details

PA3-16514

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

ChIP assay (ChIP)

1:10-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Two peptides derived from mouse CLOCK conjugated to albumin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

AB_2082567

Product Specific Information

This antibody has been tested against bacterially expressed fragment of CLOCK expressed in BL21 cells or the full length protein expressed in an in vitro translation system (reticulocyte lysate).

Target Information

This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: circadian locomoter output cycles kaput; circadian locomoter output cycles kaput protein; circadian locomotor output cycles kaput; class E basic helix-loop-helix protein 8; clock homolog; KIAA0334; rCLOCK; transcription factor; unnamed protein product

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Gene Aliases: 5330400M04Rik; bHLHe8; KAT13D

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UniProt ID: (Human) O15516, (Rat) Q9WVS9, (Mouse) O08785

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Entrez Gene ID: (Human) 9575, (Rat) 60447, (Mouse) 12753

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding histone acetyltransferase activity protein binding chromatin DNA binding sequence-specific DNA binding protein dimerization activity peptide-lysine-N-acetyltransferase activity E-box binding sequence-specific double-stranded DNA binding

Process(es)

DNA damage checkpoint chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein acetylation spermatogenesis female pregnancy circadian rhythm response to light stimulus entrainment of circadian clock gene expression regulation of gene expression circadian regulation of gene expression regulation of hair cycle regulation of circadian rhythm positive regulation of circadian rhythm proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of inflammatory response regulation of insulin secretion response to redox state cellular response to ionizing radiation regulation of type B pancreatic cell development negative regulation of glucocorticoid receptor signaling pathway signal transduction photoperiodism

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CLOCK Polyclonal Antibody

CLOCK Antibody (PA3-16514) in WB

Product Details

PA3-16514

Western Blot (WB)

ChIP assay (ChIP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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