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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • DDAH1 Antibodies

          Invitrogen

          DDAH1 Polyclonal Antibody

          Advanced Verification
          1 Published Figure
          2 References
          View all (20) DDAH1 antibodies

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          Cite DDAH1 Polyclonal Antibody

          • Antibody Testing Data (8)
          • Published Figures (1)
          • Advanced Verification (1)
          DDAH1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          DDAH1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          DDAH1 Antibody (PA5-52278) in ICC/IF

          Immunofluorecent analysis of DDAH1 in human cell line U-2 OS using DDAH1 Polyclonal Antibody (Product # PA5-52278). Staining shows localization to plasma membrane and cytosol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          DDAH1 Antibody in Immunocytochemistry (ICC/IF)
          DDAH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DDAH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DDAH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DDAH1 Antibody in Immunohistochemistry (IHC)
          DDAH1 Antibody in Immunohistochemistry (IHC)
          DDAH1 Antibody in Western Blot (WB)
          DDAH1 Antibody in Western Blot (WB)
          DDAH1 Antibody in Western Blot (WB)
          DDAH1 Antibody
          DDAH1 Polyclonal Antibody

          Product Details

          PA5-52278

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.04-0.4 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:500-1:1,000
          -

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human DDAH1. Recombinant protein control fragment (Product #RP-88775).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.20 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2640441

          Product Specific Information

          Immunogen sequence: SKRTNQRGAE ILADTFKDYA VSTVPVADGL HLKSFCSMAG PNLIAIGSSE SAQKALKIMQ QMSDHRYDKL TVPDDIAANC IYLNIPNKGH VLLHRTPEEY PESAKVYEKL KDHMLIPVSM SELEKVDGLL T

          Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.

          Target Information

          This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DDAH-1; DDAHI; Dimethylargininase-1; epididymis secretory protein Li 16; FLJ21264; FLJ25539; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; NG, NG-dimethylarginine dimethylaminohydrolase; NG,NG dimethylarginine dimethylaminohydrolase; RP4-621F18.1

          View more View less

          Gene Aliases: 2410006N07Rik; 2510015N06Rik; AI987801; AW050362; DDAH; DDAH1; HEL-S-16

          View more View less

          UniProt ID: (Human) O94760, (Mouse) Q9CWS0, (Rat) O08557

          View more View less

          Entrez Gene ID: (Human) 23576, (Mouse) 69219, (Rat) 64157

          View more View less

          Function(s)
          catalytic activity dimethylargininase activity amino acid binding metal ion binding zinc ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines hydrolase
          Process(es)
          citrulline metabolic process regulation of systemic arterial blood pressure arginine catabolic process nitric oxide mediated signal transduction positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis regulation of nitric-oxide synthase activity arginine metabolic process nitric oxide biosynthetic process protein nitrosylation
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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