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Invitrogen
SMCR8 Polyclonal Antibody
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Product Details
PA5-54449
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human SMCR8. Recombinant protein control fragment (Product #RP-93456).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SQASTTSNPD ESADTDLYTC RPAYTPKLIK AKSTKCFDKK LKTLEELCDT EYFTQTLAQL SHIEHMFRGD LCYLLTSQID RALLKQQHIT NFLFEDFVEV DDRMVEKQES IPSKPSQD
Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 86%.
Target Information
Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity. Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates. In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ34716; Guanine nucleotide exchange protein SMCR8; Smith-Magenis syndrome chromosomal region candidate gene 8 protein; Smith-Magenis syndrome chromosome region, candidate 8; unnamed protein product
Gene Aliases: DENND8A; SMCR8
UniProt ID: (Human) Q8TEV9
Entrez Gene ID: (Human) 140775
autophagy
regulation of autophagy
negative regulation of gene expression
negative regulation of macroautophagy
positive regulation of TOR signaling
negative regulation of exocytosis
negative regulation of immune response
positive regulation of autophagosome maturation
negative regulation of autophagosome assembly
regulation of TORC1 signaling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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