Western blot analysis of C6orf150 in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using a C6orf150 Polyclonal Antibody (Product # PA5-56820). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

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cGAS Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Rh30 cells were transfected with, control siRNA, C6orf150 siRNA probe #1, and a decrease in signal intensity was observed in Western Blot application using Anti-C6orf150 Polyclonal Antibody (Product # PA5-56820) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

Immunogen image

Immunogen Image cGAS Polyclonal Antibody

Product Details

PA5-56820

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human C6orf150. Recombinant protein control fragment (Product #RP-94922).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

20 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2639106

Product Specific Information

Immunogen sequence: RKQLRLKPFY LVPKHAKEGN GFQEETWRLS FSHIEKEILN NHGKSKTCCE NKEEKCCRKD CLKLMKYLLE QLKERF

Highest antigen sequence identity to the following orthologs: Mouse - 75%, Rat - 51%.

Target Information

cGAS (Cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of 2', 5' phosphodiester linkage at the GpA step and the formation of 3', 5' phosphodiester linkage at the ApG step. This produces c[G(2',5')pA(3'5')p] responses. cGAS also binds cytosolic DNA directly, leading to the activation and synthesis of cGAMP. Then, a second messenger binds to and activates TMEM173/STING thereby triggering type-I interferon production. cGAS is also involved in innate immune sensorship of infection, detection of DNA from bacteria, response to cellular stress, DNA damage, and regulation of cellular senescence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 2'3'-cGAMP synthase; C6orf150; C6orf150; cGAS is a cytosolic DNA sensor that induces interferons by producing the second messenger cGAMP; cGAMP synthase; Cyclic GMP-AMP synthase; DANGER family member 4; h-cGAS; Mab-21 domain containing 1; Mab-21 domain-containing protein 1; MB21D1; protein MB21D1; unnamed protein product

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Gene Aliases: C6orf150; CGAS; D4; h-cGAS; MB21D1

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UniProt ID: (Human) Q8N884

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Entrez Gene ID: (Human) 115004

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Function(s)

nucleotide binding DNA binding chromatin binding double-stranded DNA binding protein binding ATP binding GTP binding phosphatidylinositol-4,5-bisphosphate binding lipid binding transferase activity nucleotidyltransferase activity nucleosome binding protein homodimerization activity metal ion binding cyclic-GMP-AMP synthase activity molecular condensate scaffold activity poly-ADP-D-ribose modification-dependent protein binding

Process(es)

activation of innate immune response pattern recognition receptor signaling pathway positive regulation of defense response to virus by host immune system process regulation of immunoglobulin production cytoplasmic pattern recognition receptor signaling pathway DNA repair cellular response to DNA damage stimulus determination of adult lifespan cAMP-mediated signaling cGMP-mediated signaling signal transduction involved in regulation of gene expression regulation of type I interferon production positive regulation of type I interferon production paracrine signaling innate immune response negative regulation of DNA repair regulation of immune response regulation of T cell activation defense response to virus cellular response to exogenous dsRNA cGAS/STING signaling pathway negative regulation of cGAS/STING signaling pathway positive regulation of cytokine production involved in inflammatory response negative regulation of double-strand break repair via homologous recombination positive regulation of cellular senescence

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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cGAS Polyclonal Antibody

cGAS Antibody (PA5-56820) in WB

Product Details

PA5-56820

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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