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Invitrogen
SDHA Polyclonal Antibody
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FIGURE: 1 / 6
SDHA Antibody (PA5-66693) in ICC/IF
Immunofluorescence analysis of SDHA was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SDHA Polyclonal Antibody (Product # PA5-66693) at 1 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) (1:20... View More
Product Details
PA5-66693
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human SDHA. Recombinant protein control fragment (Product #RP-105321).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: VLRHVNGQDQI VPGLYACGEA ACASVHGANR LGANSLLDLV VFGRACALSI EESCRPGDKV PPIKPNAGEE S
Highest antigen sequence identity to the following orthologs - mouse 94%, rat 96%.
Target Information
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: flavoprotein; Flavoprotein subunit of complex II; Fp; Malate dehydrogenase [quinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex, subunit A, flavoprotein (Fp); unnamed protein product
Gene Aliases: CMD1GG; FP; MC2DN1; NDAXOA; PGL5; PPGL5; SDH1; SDH2; SDHA; SDHF
UniProt ID: (Human) P31040
Entrez Gene ID: (Human) 6389
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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