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          • Primary Antibodies ›
          • NOTCH1 Antibodies

          Invitrogen

          NOTCH1 Polyclonal Antibody

          View all (50) NOTCH1 antibodies

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          Cite NOTCH1 Polyclonal Antibody

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          • Antibody Testing Data (1)
          NOTCH1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          NOTCH1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          NOTCH1 Antibody (PA5-84750) in ICC/IF

          Immunofluorescent analysis of NOTCH1 in human cell line HaCaT using NOTCH1 Polyclonal Antibody (Product # PA5-84750). Staining shows localization to nucleoplasm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          NOTCH1 Antibody in Immunocytochemistry (ICC/IF)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          NOTCH1 Polyclonal Antibody

          Product Details

          PA5-84750

          Applications
          Tested Dilution
          Publications

          Immunocytochemistry (ICC/IF)

          0.25-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein corresponding to Human NOTCH1. Recombinant protein control fragment (Product #RP-107550).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Amount

          20 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 40% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2791900

          Product Specific Information

          Immunogen sequence: GGSTSLNGQC EWLSRLQSGM VPNQYNPLRG SVAPGPLSTQ APSLQHGMVG PLHSSLAASA LSQMMSYQGL PSTRLATQPH LVQTQQVQPQ NLQMQQQNL

          Highest antigen sequence indentity to the following orthologs: Mouse - 75%, Rat - 75%.

          Target Information

          The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: notch 1; Notch homolog 1, translocation-associated; NOTCH1; RP23-306D20.12; RP23-306D20.12-001; TAN1; translocation-associated notch protein TAN-1; unnamed protein product

          View more View less

          Gene Aliases: AOS5; AOVD1; hN1; TAN1

          View more View less

          UniProt ID: (Human) P46531

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          Entrez Gene ID: (Human) 4851

          View more View less

          Function(s)
          chromatin binding transcription coactivator activity enzyme inhibitor activity transmembrane signaling receptor activity Notch binding calcium ion binding protein binding enzyme binding chromatin DNA binding signaling receptor activity identical protein binding transcription regulator activator activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter luteolysis epithelial to mesenchymal transition heart looping positive regulation of neuroblast proliferation outflow tract morphogenesis endocardium development endocardium morphogenesis atrioventricular node development coronary vein morphogenesis aortic valve morphogenesis atrioventricular valve morphogenesis coronary sinus valve morphogenesis pulmonary valve morphogenesis mitral valve formation epithelial to mesenchymal transition involved in endocardial cushion formation endocardial cushion morphogenesis cardiac chamber formation cardiac ventricle morphogenesis cardiac atrium morphogenesis cardiac right atrium morphogenesis cardiac left ventricle morphogenesis ventricular trabecula myocardium morphogenesis growth involved in heart morphogenesis regulation of cardioblast proliferation cell migration involved in endocardial cushion formation negative regulation of extracellular matrix constituent secretion pericardium morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter protein import into nucleus immune response Notch signaling pathway positive regulation of transcription of Notch receptor target spermatogenesis determination of left/right symmetry axon guidance heart development positive regulation of cell proliferation negative regulation of cell proliferation gene expression negative regulation of cardiac muscle hypertrophy positive regulation of gene expression negative regulation of gene expression negative regulation of cardiac muscle cell apoptotic process positive regulation of epithelial to mesenchymal transition negative regulation of cell-substrate adhesion negative regulation of myotube differentiation mesenchymal cell development cell differentiation in spinal cord cell differentiation protein catabolic process negative regulation of ossification regulation of cell migration positive regulation of cell migration positive regulation of BMP signaling pathway negative regulation of BMP signaling pathway organ regeneration response to muramyl dipeptide response to lipopolysaccharide negative regulation of collagen biosynthetic process tube formation cellular response to vascular endothelial growth factor stimulus regulation of cell proliferation tissue regeneration negative regulation of catalytic activity positive regulation of viral genome replication positive regulation of endothelial cell differentiation negative regulation of myoblast differentiation negative regulation of neuron differentiation negative regulation of osteoblast differentiation positive regulation of glial cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade positive regulation of Ras protein signal transduction astrocyte differentiation oligodendrocyte differentiation positive regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation homeostasis of number of cells within a tissue negative regulation of neurogenesis regulation of developmental process positive regulation of smooth muscle cell differentiation cardiac muscle tissue morphogenesis cardiac muscle cell proliferation positive regulation of cardiac muscle cell proliferation negative regulation of glial cell proliferation cilium morphogenesis cardiac epithelial to mesenchymal transition negative regulation of cell adhesion molecule production cardiac muscle cell myoblast differentiation cardiac septum morphogenesis ventricular septum morphogenesis arterial endothelial cell differentiation venous endothelial cell differentiation cardiac vascular smooth muscle cell development endocardial cell differentiation vasculogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis regulation of cell adhesion involved in heart morphogenesis heart trabecula morphogenesis positive regulation of cardiac epithelial to mesenchymal transition negative regulation of biomineral tissue development positive regulation of ERK1 and ERK2 cascade left/right axis specification cellular response to tumor cell cellular response to follicle-stimulating hormone stimulus cellular response to hypoxia distal tubule development collecting duct development glomerular mesangial cell development T-helper 17 type immune response negative regulation of cell migration involved in sprouting angiogenesis neuronal stem cell population maintenance interleukin-17-mediated signaling pathway chemical synaptic transmission, postsynaptic negative regulation of cold-induced thermogenesis regulation of extracellular matrix assembly positive regulation of apoptotic process involved in morphogenesis negative regulation of cell-cell adhesion mediated by cadherin negative regulation of cell proliferation involved in heart morphogenesis negative regulation of stem cell differentiation negative regulation of anoikis negative regulation of pro-B cell differentiation negative regulation of endothelial cell chemotaxis
          It has to be done as per old AB suggested Products section.

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