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Invitrogen
Phospho-JunD (Ser255) Polyclonal Antibody
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FIGURE: 1 / 20
Phospho-JunD (Ser255) Antibody (PA5-104821) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-JunD (Ser255) in human bladder cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-JunD (Ser255) polyclonal antibody (Product # PA5-104821) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-104821
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human JUND(Accession P17535), corresponding to amino acid residues around phosphorylated Ser255.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of JunD only when phosphorylated at Serine 255.
Target Information
JunD is a transcription factor which is coded by JunD gene. JunD transcriptional activity is modulated by phosphorylation in response to cellular stress via the c-Jun N terminal kinase (JNK)/Stress-Activated Protein Kinase (SAPK) family of protein kinase. Jun proteins are capable of forming dimers with Fos, ATF and CREB family transcription factors to form the AP-1 complex that differentially regulates a variety of target genes involved in cellular growth, proliferation, differentiation and apoptosis. Also, JunD plays an important role in metabolism via modulation of IGF-1 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activator protein 1; AP1; JUN D; jun D proto-oncogene; Jun proto-oncogene related gene d; Jun proto-oncogene related gene d1; Oncogene Jun-D; transcription factor AP-1 subunit JunD; transcription factor jun-D; unnamed protein product
Gene Aliases: AP-1; Jund1
UniProt ID: (Human) P17535, (Mouse) P15066, (Rat) P52909
Entrez Gene ID: (Human) 3727, (Mouse) 16478, (Rat) 24518
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription cofactor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ligand-dependent nuclear receptor binding
enzyme binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
transcription coactivator activity
negative regulation of transcription from RNA polymerase II promoter
osteoblast development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
circadian rhythm
response to cold
response to water deprivation
response to light stimulus
response to mechanical stimulus
gene expression
negative regulation of cardiac muscle hypertrophy
response to lipopolysaccharide
positive regulation of peroxisome proliferator activated receptor signaling pathway
regulation of cell proliferation
positive regulation of macrophage activation
response to peptide hormone
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
response to mercury ion
response to steroid hormone
epithelial cell proliferation
negative regulation of epithelial cell proliferation
regulation of cell cycle
cellular response to calcium ion
cellular response to fatty acid
cellular response to hypoxia
response to forskolin
cellular response to polyamine macromolecule
regulation of transcription from RNA polymerase II promoter
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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