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Invitrogen
Phospho-MST2 (Ser316) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-MST2 (Ser316) Antibody (PA5-105065) in WB
Western blot analysis of Phospho-MST2 (Ser316) in extracts from Hela cells (H2O2 treatment). Samples were incubated with Phospho-MST2 (Ser316) polyclonal antibody (Product # PA5-105065). The lane on the left was treated with blocking peptide.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human STK3(Accession Q13188), corresponding to amino acid residues around phosphorylated Ser316.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of STK3 only when phosphorylated at Ser316.
Target Information
STK3 (MST2) is a STE kinase family member that acts upstream of the MAP kinase signaling pathways, and is known to be activated by the proapoptotic stimuli staurosporine and FAS ligand. STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; hippo homolog; KB-1458E12.1; mammalian STE20-like protein kinase 2; MST-2; protein kinase; protein kinase homolog; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine-protein kinase Krs-1; STE20-like kinase MST2; unnamed protein product
Gene Aliases: 0610042I06Rik; KRS1; mess1; MST; MST2; Mst3; Ste20
UniProt ID: (Human) Q13188, (Mouse) Q9JI10, (Rat) O54748
Entrez Gene ID: (Human) 6788, (Mouse) 56274, (Rat) 65189
protein phosphorylation
protein import into nucleus
apoptotic process
signal transduction
regulation of gene expression
hippo signaling
positive regulation of hippo signaling
intracellular signal transduction
positive regulation of apoptotic process
regulation of MAPK cascade
negative regulation of growth
protein stabilization
protein tetramerization
negative regulation of cell growth involved in contact inhibition
protein localization to centrosome
negative regulation of canonical Wnt signaling pathway
neural tube formation
endocardium development
central nervous system development
negative regulation of cell proliferation
positive regulation of fat cell differentiation
positive regulation of JNK cascade
negative regulation of organ growth
centrosome separation
positive regulation of protein kinase B signaling
primitive hemopoiesis
cell differentiation involved in embryonic placenta development
regulation of cell differentiation involved in embryonic placenta development
hepatocyte apoptotic process
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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