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Invitrogen

Phospho-MiTF (Ser516) Polyclonal Antibody

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Phospho-MiTF (Ser516) Polyclonal Antibody

Product Details

PA5-105415

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MITF(Accession O75030), corresponding to amino acid residues around phosphorylated Ser516.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816843

Product Specific Information

Antibody detects endogenous levels of MITF only when phosphorylated at Ser516.

Target Information

Mi is a basic helix-loop-helix-leucine zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHe32; black eyed white; Class E basic helix-loop-helix protein 32; melanogenesis associated transcription factor; microphtalmia-associated transcription factor; microphthalmia; Microphthalmia-associated transcription factor; unnamed protein product

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Gene Aliases: BCC2; BHLHE32; Bw; CMM8; COMMAD; Gsfbcc2; MI; MITF; MITF-A; Vit; Vitiligo; Wh; WS2; WS2A

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UniProt ID: (Human) O75030, (Rat) O88368, (Mouse) Q08874

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Entrez Gene ID: (Human) 4286, (Rat) 25094, (Mouse) 17342

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein dimerization activity E-box binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter regulation of gene expression positive regulation of gene expression Wnt signaling pathway cell differentiation osteoclast differentiation melanocyte differentiation negative regulation of cell migration regulation of cell proliferation camera-type eye development negative regulation of apoptotic process pigmentation cell fate commitment regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter bone remodeling protein-containing complex assembly melanocyte apoptotic process positive regulation of DNA-templated transcription, initiation regulation of RNA biosynthetic process transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly multicellular organism development regulation of apoptotic process canonical Wnt signaling pathway involved in negative regulation of apoptotic process mast cell migration
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