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Invitrogen

Phospho-LCK (Tyr505) Polyclonal Antibody

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Phospho-LCK (Tyr505) Polyclonal Antibody

Product Details

PA5-105467

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human LCK around the phosphorylation site of Tyr505.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816895

Product Specific Information

Antibody detects endogenous levels of Lck only when phosphorylated at Tyr505.

Target Information

LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte protein tyrosine kinase; lymphocyte-specific protein tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56-LCK; Protein YT16; Proto-oncogene Lck; Proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; Tyrosine-protein kinase Lck; unnamed protein product

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Gene Aliases: Hck-3; IMD22; LCK; Lck1; Lcktkr; LSK; Lsk-t; Lskt; p56; p56Lck; pp58lck; YT16

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UniProt ID: (Human) P06239, (Rat) Q01621, (Mouse) P06240

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Entrez Gene ID: (Human) 3932, (Rat) 313050, (Mouse) 16818

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Function(s)
nucleotide binding phosphotyrosine binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein serine/threonine phosphatase activity receptor binding protein binding ATP binding phospholipase activator activity kinase activity transferase activity protein kinase binding protein phosphatase binding SH2 domain binding T cell receptor binding CD4 receptor binding CD8 receptor binding identical protein binding phospholipase binding phosphatidylinositol 3-kinase binding ATPase binding protein antigen binding glycoprotein binding antigen binding protein C-terminus binding protein complex binding transferase activity, transferring phosphorus-containing groups
Process(es)
protein phosphorylation cellular zinc ion homeostasis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway aging response to mechanical stimulus response to metal ion response to zinc ion positive regulation of gene expression dephosphorylation peptidyl-tyrosine phosphorylation T cell differentiation peptidyl-tyrosine autophosphorylation regulation of cell proliferation response to drug positive regulation of tyrosine phosphorylation of Stat5 protein response to hydrogen peroxide regulation of apoptotic process innate immune response positive regulation of gamma-delta T cell differentiation protein autophosphorylation B cell receptor signaling pathway regulation of T cell receptor signaling pathway positive regulation of T cell receptor signaling pathway positive regulation of T cell activation release of sequestered calcium ion into cytosol positive regulation of uterine smooth muscle contraction cellular response to peptide hormone stimulus positive regulation of intrinsic apoptotic signaling pathway phosphorylation immune response-activating cell surface receptor signaling pathway response to xenobiotic stimulus hemopoiesis platelet activation T cell costimulation positive regulation of heterotypic cell-cell adhesion intracellular signal transduction Fc-gamma receptor signaling pathway T cell activation gamma-delta T cell differentiation positive regulation of MAPK cascade regulation of regulatory T cell differentiation T cell receptor signaling pathway leukocyte migration positive regulation of multicellular organismal process regulation of lymphocyte activation CD27 signaling pathway positive regulation of leukocyte cell-cell adhesion
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