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Invitrogen
Phospho-WASP (Tyr291) Polyclonal Antibody
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FIGURE: 1 / 8
Phospho-WASP (Tyr291) Antibody (PA5-105572) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-WASP (Tyr291) in mouse lung tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-WASP (Tyr291) polyclonal antibody (Product # PA5-105572) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-105572
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human WAS(Accession P42768), corresponding to amino acid residues around phosphorylated Tyr291.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of WASP only when phosphorylated at Tyr291.
Target Information
The Wiskott-Aldrich syndrome family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Actin nucleation-promoting factor WAS; eczema-thrombocytopenia; thrombocytopenia 1 (X-linked); WAS; WASp; Wiskott-Aldrich syndrome homolog; Wiskott-Aldrich syndrome protein; Wiskott-Aldrich syndrome protein homolog
Gene Aliases: IMD2; SCNX; THC; THC1; U42471; WAS; WASP; WASPA
UniProt ID: (Human) P42768, (Mouse) P70315
Entrez Gene ID: (Human) 7454, (Rat) 317371, (Mouse) 22376
regulation of T cell antigen processing and presentation
defense response
immune response
actin filament organization
Rho protein signal transduction
blood coagulation
regulation of actin polymerization or depolymerization
actin polymerization or depolymerization
epidermis development
regulation of lamellipodium assembly
endosomal transport
actin filament polymerization
actin filament-based movement
Cdc42 protein signal transduction
T cell activation
positive regulation of transcription from RNA polymerase II promoter
regulation of stress fiber assembly
negative regulation of stress fiber assembly
protein-containing complex assembly
cellular response to interferon-gamma
positive regulation of double-strand break repair via homologous recombination
negative regulation of cell motility
regulation of double-strand break repair via nonhomologous end joining
biological_process
positive regulation of Arp2/3 complex-mediated actin nucleation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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