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Invitrogen
MKL1 Polyclonal Antibody
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FIGURE: 1 / 3
MKL1 Antibody (PA5-109960) in ICC/IF
Immunofluorescence analysis of Mrtfa was performed using 70% confluent log phase BJ cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MKL1 Polyclonal Antibody (Product # PA5-109960) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-109960
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.98 mg/mL
Amount
98 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MPPLKSPAAF HEQRRSLERA RTEDYLKRKI RSRPERSELV RMHILEETSA EPSLQAKQLK LKRARLADDL NEKIAQRPGP MELVEKNILP VESSLKEAII VGQVNYPKVA DSSSFDEDSS DALSPEQPAS HESQGSVPSP LEARVSEPLL SATSASPTQV VSQLPMGRDS REMLFLAEQP PLPPPPLLPP SLTNGTTIPT AKSTPTLIKQ SQPKSASEKS QRSKKAKELK PKVKKLKYHQ YIPPDQKQDR GAPPMDSSYA KILQQQQLFL QLQILNQQQQ
Target Information
The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alternative protein MKL1; basic SAP and coiled-coil domains; basic SAP coiled-coil transcription activator; basic, SAP and coiled-coil domain; basic, SAP, and coiled-coil domain containing protein; basic, SAP, coiled-coil domain; transcriptional activator; megakaryoblastic leukemia (translocation) 1; megakaryoblastic leukemia (translocation) 1 homolog; megakaryoblastic leukemia 1 met; megakaryoblastic leukemia 1 protein; megakaryocytic acute leukemia protein; megakaryocytic acute leukemia protein homolog; mixed lineage leukemia; MKL (megakaryoblastic leukemia)/myocardin-like 1; MKL/myocardin-like protein 1; MKL1; MKL1-elongated derivative of yield protein; MRTF-A; myocardial related transcription factor A; unnamed protein product
Gene Aliases: AMKL; BSAC; FLMKL1; MAL; MELODY; MKL; MKL1; MKL1met; MRTF-A
UniProt ID: (Human) Q969V6, (Mouse) Q8K4J6
Entrez Gene ID: (Human) 57591, (Mouse) 223701, (Rat) 315151
actin cytoskeleton organization
wound healing, spreading of cells
positive regulation of transcription from RNA polymerase II promoter
smooth muscle cell differentiation
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
neuron migration
regulation of transcription from RNA polymerase II promoter
positive regulation of cardiac muscle hypertrophy
negative regulation of cardiac muscle cell apoptotic process
positive regulation of actin filament polymerization
forebrain development
neuron projection development
positive regulation of collagen biosynthetic process
negative regulation of neuron apoptotic process
positive regulation of endothelial cell differentiation
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of hepatic stellate cell migration
positive regulation of hepatic stellate cell contraction
regulation of modification of postsynaptic structure
positive regulation of hepatic stellate cell proliferation
positive regulation of hepatic stellate cell activation
negative regulation of apoptotic signaling pathway
cellular response to retinoic acid
cellular response to transforming growth factor beta stimulus
cellular response to angiotensin
response to amyloid-beta
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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