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Invitrogen
Phospho-SHC (Tyr427) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-SHC (Tyr427) Antibody (PA5-117263) in WB
Western blot analysis of Phospho-SHC (Tyr427) in extracts of A-431 cells. Samples were incubated with Phospho-SHC (Tyr427) Polyclonal antibody (Product # PA5-117263) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. A-431 cells were treated by EGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight. Lysates/proteins: 25 µg p... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-117263
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic phosphorylated peptide around Y427 of human SHC1 (NP_8921134)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.03 mg/mL
Amount
203 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-431
Immunogen sequence: PSYVN
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays. In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, in some colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ26504; OTTHUMP00000035472; RP11-307C12.1; SH2 domain protein C1; SHC (Src homology 2 domain containing) transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; unnamed protein product
Gene Aliases: p66; p66shc; SHC; SHCA
UniProt ID: (Human) P29353, (Mouse) P98083
Entrez Gene ID: (Human) 6464, (Mouse) 20416
phosphotyrosine binding
transmembrane receptor protein tyrosine kinase adaptor activity
epidermal growth factor receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
phospholipid binding
receptor tyrosine kinase binding
ephrin receptor binding
epidermal growth factor binding
protein binding, bridging
identical protein binding
macromolecular complex binding
phosphoprotein binding
protein phosphatase 2A binding
epidermal growth factor receptor signaling pathway
positive regulation of cell proliferation
insulin receptor signaling pathway
negative regulation of angiogenesis
intracellular signal transduction
defense response to bacterium
negative regulation of apoptotic process
positive regulation of MAPK cascade
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
insulin-like growth factor receptor signaling pathway
cellular response to growth factor stimulus
positive regulation of cell proliferation in bone marrow
regulation of superoxide metabolic process
angiogenesis
regulation of smooth muscle contraction
heart development
actin cytoskeleton organization
neuron differentiation
neuron projection development
regulation of cell proliferation
positive regulation of vasoconstriction
positive regulation of smooth muscle cell proliferation
B cell receptor signaling pathway
positive regulation of ERK1 and ERK2 cascade
cell-cell adhesion
response to endothelin
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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