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          • Primary Antibodies ›
          • AKT1 Antibodies

          Invitrogen

          Phospho-AKT1 (Ser129) Polyclonal Antibody

          View all (167) AKT1 antibodies

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          Cite Phospho-AKT1 (Ser129) Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          Phospho-AKT1 (Ser129) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-AKT1 (Ser129) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          Phospho-AKT1 (Ser129) Antibody (PA5-119516) in WB

          Western blot analysis of Phospho-AKT1 (Ser129) in various whole cell extracts (30 µg). Sample was separated with 7.5% SDS-PAGE, incubated with polyclonal Phospho-AKT1 (Ser129) antibody (Product # PA5-119516) at a dilution of 1:1000, and followed by HRP-conjugated anti-rabbit IgG and developed with Trident ECL plus-Enhanced. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-AKT1 (Ser129) Antibody in Western Blot (WB)
          Phospho-AKT1 (Ser129) Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Phospho-AKT1 (Ser129) Polyclonal Antibody

          Product Details

          PA5-119516

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          -
          Product Specifications

          Species Reactivity

          Human, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Carrier-protein conjugated synthetic peptide surrounding phospho Ser129 of human AKT1. The exact sequence is proprietary.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.05 mg/mL

          Amount

          105 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 1% BSA, 20% glycerol

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2913090

          Product Specific Information

          Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

          Target Information

          AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: AKT; AKT1 kinase; AKT1M; C-AKT; CAKT; murine thymoma viral (v-akt) oncogene homolog 1; PKB ALPHA; PKB beta; PKB gamma; PKB-ALPHA; PKBG; PRKBA; PROTEIN KINASE B ALPHA; PROTO-ONCOGENE C-AKT; RAC; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC-ALPHA; RAC-ALPHA SERINE/THREONINE-PROTEIN KINASE; RAC-PK-ALPHA; RAC-PK-beta; RAC-PK-gamma; serine-threonine protein kinase; thymoma viral proto-oncogene 1; unnamed protein product; V-AKT MURINE THYMOMA VIRAL ONCOGENE HOMOLOG 1; V-AKT MURINE THYMOMA VIRAL ONCOGENE-LIKE PROTEIN 1

          View more View less

          Gene Aliases: AKT; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA

          View more View less

          UniProt ID: (Human) P31749, (Rat) P47196

          View more View less

          Entrez Gene ID: (Human) 207, (Rat) 24185

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity TORC2 complex binding protein kinase C binding GTPase activating protein binding protein phosphatase 2A binding protein serine/threonine kinase binding
          Process(es)
          osteoblast differentiation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway regulation of glycogen biosynthetic process protein phosphorylation nitric oxide biosynthetic process activation-induced cell death of T cells response to oxidative stress signal transduction epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway cell proliferation insulin receptor signaling pathway response to heat response to hormone negative regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport fibroblast migration positive regulation of glucose metabolic process regulation of neuron projection development negative regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytokine-mediated signaling pathway cell differentiation positive regulation of cell growth regulation of cell migration positive regulation of cell migration T cell costimulation negative regulation of protein ubiquitination TOR signaling negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation cellular response to stress response to fluid shear stress intracellular signal transduction interleukin-18-mediated signaling pathway positive regulation of protein import into nucleus regulation of apoptotic process negative regulation of apoptotic process anoikis regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process negative regulation of Notch signaling pathway negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter nitric oxide metabolic process positive regulation of glucose import positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation positive regulation of protein metabolic process positive regulation of protein kinase B signaling excitatory postsynaptic potential response to growth hormone mammary gland epithelial cell differentiation response to UV-A protein localization to mitochondrion response to growth factor cellular response to epidermal growth factor stimulus maintenance of protein location in mitochondrion cellular response to rapamycin negative regulation of release of cytochrome c from mitochondria vascular endothelial cell response to laminar fluid shear stress regulation of postsynapse organization regulation of tRNA methylation cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator negative regulation of cilium assembly negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of protein maturation negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of PERK-mediated unfolded protein response positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface regulation of type B pancreatic cell development negative regulation of lymphocyte migration negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway maternal placenta development response to ischemia glycogen metabolic process glycogen biosynthetic process glucose metabolic process translation inflammatory response cellular response to DNA damage stimulus smoothened signaling pathway germ cell development apoptotic mitochondrial changes gene expression positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of mitochondrial membrane potential response to muscle activity protein ubiquitination spinal cord development lateral motor column neuron differentiation oogenesis stage cell projection organization protein catabolic process regulation of myelination lipopolysaccharide-mediated signaling pathway response to nutrient levels response to food peripheral nervous system myelin maintenance regulation of protein localization negative regulation of superoxide anion generation cellular response to heat cellular response to vascular endothelial growth factor stimulus cellular response to platelet-derived growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis positive regulation of apoptotic process negative regulation of cell size positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of organ growth protein autophosphorylation protein stabilization striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development cellular response to mechanical stimulus cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia cellular hyperosmotic salinity response cellular response to sorbitol cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis negative regulation of calcium import into the mitochondrion cellular response to peptide regulation of aerobic respiration
          It has to be done as per old AB suggested Products section.

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