Western blot analysis of 14-3-3 zeta in extracts of various cell lines. Samples were incubated with 14-3-3 zeta Polyclonal antibody (Product # PA5-120298) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image 14-3-3 zeta Polyclonal Antibody

Product Details

PA5-120298

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

ELISA (ELISA)

1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human YWHAZ (NP_001129171.1).

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.08 mg/mL

208 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

AB_2913870

Product Specific Information

The target is usually found in the following locations: Cytoplasm, Melanosome.

Immunogen sequence: MDKNELVQKA KLAEQAERYD DMAACMKSVT EQGAELSNEE RNLLSVAYKN VVGARRSSWR VVSSIEQKTE GAEKKQQMAR EYREKIETEL RDICNDVLSL

Target Information

At least seven isoforms comprise the highly conserved 14-3-3 family of homo- and heterodimeric proteins that are abundantly expressed in all eukaryotic cells. Although more than seven isoforms of 14-3-3 have been described, some redundancies have appeared upon sequencing. The 14-3-3s are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. By interacting with Cdc25C, 14-3-3 regulates entry into the cell cycle and through interaction with Bad, prevents apoptosis. Other proteins that have been shown to bind to 14-3-3s include members of the protein kinase C family, Cbl, IRS-1, polyoma middle-T antigen, nitrate reductase, S-raf and the IGF-1 receptor. Detection of 14-3-3 proteins in cerebrospinal fluid has been shown to be useful in the differential diagnosis of Creutzfeldt-Jakob disease and other prion diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 14-3-3 delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; 1433Z; 143Z; epididymis luminal protein 4; epididymis secretory protein Li 3; epididymis secretory protein Li 93; KCIP-1; mitochondrial import stimulation factor S1 subunit; phospholipase A2; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; SEZ-2; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; unnamed protein product

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Gene Aliases: 1110013I11Rik; 14-3-3-zeta; 14-3-3z; 14-3-3zeta; HEL-S-3; HEL-S-93; HEL4; KCIP-1; POPCHAS; YWHAD

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UniProt ID: (Human) P63104, (Mouse) P63101, (Rat) P63102

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Entrez Gene ID: (Human) 7534, (Mouse) 22631, (Rat) 25578

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Function(s)

RNA binding protein binding protein kinase binding protein phosphatase binding protein domain specific binding ubiquitin protein ligase binding identical protein binding ion channel binding cadherin binding phosphoserine binding DNA-binding transcription factor binding protein sequestering activity macromolecular complex binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter angiogenesis respiratory system process protein phosphorylation signal transduction synaptic target recognition protein localization lung development regulation of protein stability tube formation cellular response to glucose starvation negative regulation of apoptotic process negative regulation of innate immune response establishment of Golgi localization regulation of ERK1 and ERK2 cascade regulation of synapse maturation Golgi reassembly negative regulation of protein localization to nucleus negative regulation of TORC1 signaling histamine secretion by mast cell protein targeting lysosome organization response to xenobiotic stimulus cellular response to nutrient levels TORC1 signaling regulation of programmed cell death establishment of protein localization ERK1 and ERK2 cascade protein localization to mitochondrion antibacterial innate immune response regulation of lysosome organization

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14-3-3 zeta Polyclonal Antibody

14-3-3 zeta Antibody (PA5-120298) in WB

Product Details

PA5-120298

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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