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          • Primary Antibodies ›
          • DJ-1 Antibodies

          Invitrogen

          DJ-1 Polyclonal Antibody

          Advanced Verification
          2 References
          View all (33) DJ-1 antibodies

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          Cite DJ-1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (6)
          • Advanced Verification (1)
          DJ-1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          DJ-1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          DJ-1 Antibody (PA5-13404) in ICC/IF

          Immunofluorescence analysis of DJ1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with DJ1 Polyclonal Antibody (Product # PA5-13404) at 1:50 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dil... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          DJ-1 Antibody in Immunocytochemistry (ICC/IF)
          DJ-1 Antibody in Immunocytochemistry (ICC/IF)
          DJ-1 Antibody in Western Blot (WB)
          DJ-1 Antibody in Western Blot (WB)
          DJ-1 Antibody in Western Blot (WB)
          DJ-1 Antibody in Western Blot (WB)
          DJ-1 Antibody
          DJ-1 Polyclonal Antibody

          Product Details

          PA5-13404

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:10-1:50
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DJ-1
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2 mg/mL

          Amount

          800 µg

          Purification

          Ammonium sulfate precipitation, Size-exclusion - Dialysis

          Storage buffer

          PBS, pH 7.4

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2160112

          Product Specific Information

          This antibody is predicted to react with bovine and zebrafish based on sequence homology.

          Target Information

          The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CTA-215D11.1; DJ-1; early onset 7; epididymis secretory sperm binding protein Li 67p; FLJ27376; FLJ34360; FLJ92274; Maillard deglycase; Oncogene DJ1; OTTHUMP00000001350; OTTHUMP00000001351; PARK7 GENE; PARK7/DJ1; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; Parkinson disease protein 7 homolog; parkinson protein 7; Parkinsonism-associated deglycase; protein deglycase DJ-1; Protein DJ-1; Protein/nucleic acid deglycase DJ-1; RNA-binding protein regulatory subunit; unnamed protein product

          View more View less

          Gene Aliases: DJ-1; DJ1; GATD2; HEL-S-67p; PARK7

          View more View less

          UniProt ID: (Human) Q99497, (Mouse) Q99LX0

          View more View less

          Entrez Gene ID: (Human) 11315, (Mouse) 57320

          View more View less

          Function(s)
          core promoter sequence-specific DNA binding double-stranded DNA binding single-stranded DNA binding transcription coactivator activity RNA binding mRNA binding receptor binding copper ion binding protein binding enzyme activator activity peptidase activity superoxide dismutase copper chaperone activity oxidoreductase activity, acting on peroxide as acceptor hydrolase activity oxygen sensor activity enzyme binding kinase binding cytokine binding peptidase inhibitor activity receptor activator activity tyrosine 3-monooxygenase activator activity L-dopa decarboxylase activator activity protein deglycase activity identical protein binding protein homodimerization activity small protein activating enzyme binding ubiquitin-like protein conjugating enzyme binding cadherin binding mercury ion binding androgen receptor binding peroxiredoxin activity ubiquitin-protein transferase inhibitor activity scaffold protein binding DNA-binding transcription factor binding cupric ion binding cuprous ion binding ubiquitin-specific protease binding glyoxalase (glycolic acid-forming) activity histone deacetylase activity transcription factor binding protein-N-terminal asparagine amidohydrolase activity UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity CoA hydrolase activity iprodione amidohydrolase activity (3,5-dichlorophenylurea)acetate amidohydrolase activity glyoxalase III activity 4'-(2-hydroxyisopropyl)phenylurea amidohydrolase activity didemethylisoproturon amidohydrolase activity N-isopropylacetanilide amidohydrolase activity N-cyclohexylformamide amidohydrolase activity 2-oxoglutaryl-CoA thioesterase activity 2,4,4-trimethyl-3-oxopentanoyl-CoA thioesterase activity isonicotinic acid hydrazide hydrolase activity cis-aconitamide amidase activity gamma-N-formylaminovinylacetate hydrolase activity 3-isopropylbut-3-enoyl-CoA thioesterase activity N2-acetyl-L-lysine deacetylase activity O-succinylbenzoate synthase activity indoleacetamide hydrolase activity N-acetylcitrulline deacetylase activity glutaryl-CoA hydrolase activity N-acetylgalactosamine-6-phosphate deacetylase activity acyl-CoA hydrolase activity diacetylchitobiose deacetylase activity chitooligosaccharide deacetylase activity repressing transcription factor binding
          Process(es)
          negative regulation of protein phosphorylation synaptic transmission, dopaminergic negative regulation of protein kinase activity proteolysis protein deglycosylation autophagy inflammatory response response to oxidative stress mitochondrion organization single fertilization cell proliferation adult locomotory behavior detoxification of copper ion positive regulation of gene expression negative regulation of gene expression enzyme active site formation via L-cysteine sulfinic acid methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione lactate biosynthetic process negative regulation of protein ubiquitination negative regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of interleukin-8 production positive regulation of peptidyl-serine phosphorylation negative regulation of protein sumoylation cellular response to oxidative stress cellular response to reactive oxygen species cellular response to glyoxal negative regulation of protein catabolic process response to hydrogen peroxide hydrogen peroxide metabolic process negative regulation of apoptotic process regulation of neuron apoptotic process negative regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter glycolate biosynthetic process negative regulation of protein export from nucleus regulation of inflammatory response detoxification of mercury ion protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of ubiquitin-protein transferase activity dopamine uptake involved in synaptic transmission regulation of mitochondrial membrane potential membrane depolarization oxidation-reduction process membrane hyperpolarization negative regulation of cell death regulation of androgen receptor signaling pathway cellular response to hydrogen peroxide positive regulation of protein homodimerization activity positive regulation of protein localization to nucleus negative regulation of neuron death positive regulation of superoxide dismutase activity negative regulation of protein acetylation positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of mitochondrial electron transport, NADH to ubiquinone negative regulation of protein K48-linked deubiquitination negative regulation of TRAIL-activated apoptotic signaling pathway positive regulation of pyrroline-5-carboxylate reductase activity positive regulation of tyrosine 3-monooxygenase activity positive regulation of dopamine biosynthetic process glyoxal catabolic process positive regulation of L-dopa biosynthetic process positive regulation of L-dopa decarboxylase activity negative regulation of oxidative stress-induced cell death negative regulation of oxidative stress-induced neuron death negative regulation of hydrogen peroxide-induced cell death negative regulation of hydrogen peroxide-induced neuron death negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway positive regulation of reactive oxygen species biosynthetic process negative regulation of ubiquitin-specific protease activity positive regulation of oxidative phosphorylation uncoupler activity positive regulation of transcription regulatory region DNA binding positive regulation of androgen receptor activity negative regulation of extrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of acute inflammatory response to antigenic stimulus DNA repair cellular response to DNA damage stimulus Ras protein signal transduction methylglyoxal metabolic process removal of superoxide radicals insulin secretion protein repair androgen receptor signaling pathway positive regulation of protein complex assembly regulation of TRAIL production glucose homeostasis positive regulation of protein kinase B signaling detoxification of hydrogen peroxide methylglyoxal catabolic process to lactate detection of oxidative stress guanine deglycation guanine deglycation, methylglyoxal removal guanine deglycation, glyoxal removal cellular detoxification of aldehyde cellular detoxification of methylglyoxal regulation of synaptic vesicle endocytosis negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway regulation of fibril organization negative regulation of death-inducing signaling complex assembly glyoxal metabolic process regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of reactive oxygen species biosynthetic process positive regulation of mitophagy negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway positive regulation of reactive oxygen species metabolic process
          It has to be done as per old AB suggested Products section.

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