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Invitrogen
Phospho-RNA pol II CTD (Ser2, Ser5) Polyclonal Antibody
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FIGURE: 1 / 3
Phospho-RNA pol II CTD (Ser2, Ser5) Antibody (PA5-17563) in IHC (P)
Immunohistochemistry was performed on paraffin-embedded human lung carcinoma tissue. To expose target proteins, antigen retrieval was performed by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10 minutes. Following antigen retrieval, endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes, tissues were blocked in 5% normal goat serum in TBST for 1 hour at roo... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17563
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding pSer2/5 of the Rpb1 CTD heptapeptide
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
24 µg/mL
Amount
2.4 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exoribonuclease; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; Polymerase (RNA II (DNA directed), large polypeptide; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II; RNA polymerase II 1; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8; unnamed protein product
Gene Aliases: 220kDa; hRPB220; hsRPB1; NEDHIB; POLR2; POLRA; RPB1; RPBh1; RpIILS; RPO2; Rpo2-1; RPOL2
UniProt ID: (Human) P24928, (Mouse) P08775
Entrez Gene ID: (Human) 5430, (Mouse) 20020, (Rat) 363633
magnesium ion binding
nucleic acid binding
DNA binding
RNA binding
DNA-directed RNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
zinc ion binding
kinase binding
ubiquitin protein ligase binding
RNA polymerase activity
microfibril binding
promoter-specific chromatin binding
core promoter sequence-specific DNA binding
transcription, RNA-templated
transcription, DNA-templated
DNA-templated transcription, termination
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
termination of RNA polymerase II transcription
positive regulation of RNA splicing
mRNA transcription from RNA polymerase II promoter
cellular response to oxygen levels
regulation of transcription, DNA-templated
positive regulation of gene expression
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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