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Invitrogen

PKA alpha Polyclonal Antibody

2 References
View all (21) PKA alpha antibodies

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Product Details

PA5-21842

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Zebrafish

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151993

Product Specific Information

Recommended positive controls: HeLaS3, NIH-3T3, C2C12.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (94%), Dog (99%), Rabbit (97%), Sheep (99%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C(s); C-alpha-2; C-alpha-S; C-alpha-s; alternatively spliced isoform; MGC102831; MGC48865; PKA; PKA C-alpha; pkac; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit; unnamed protein product

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Gene Aliases: CAFD1; PKACA; PKCD; PPNAD4

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UniProt ID: (Human) P17612, (Mouse) P05132

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Entrez Gene ID: (Human) 5566, (Mouse) 18747

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Function(s)
magnesium ion binding protein kinase activity protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding potassium channel inhibitor activity protein kinase binding protein domain specific binding manganese ion binding protein kinase A regulatory subunit binding channel activator activity protein serine kinase activity histone H1-4S35 kinase activity small GTPase binding ubiquitin protein ligase binding macromolecular complex binding
Process(es)
mesoderm formation neural tube closure chromatin remodeling mRNA processing protein phosphorylation adenylate cyclase-activating G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway positive regulation of cell proliferation positive regulation of triglyceride catabolic process peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cellular potassium ion homeostasis cellular response to nutrient levels cellular response to heat insulin secretion involved in cellular response to glucose stimulus interleukin-2-mediated signaling pathway TORC1 signaling regulation of cellular respiration positive regulation of cholesterol biosynthetic process regulation of osteoblast differentiation positive regulation of gluconeogenesis negative regulation of glycolytic process negative regulation of smoothened signaling pathway protein autophosphorylation positive regulation of protein export from nucleus sperm capacitation spontaneous exocytosis of neurotransmitter positive regulation of phagocytosis modulation of synaptic transmission negative regulation of meiotic cell cycle regulation of cell cycle regulation of synaptic transmission, glutamatergic regulation of proteasomal protein catabolic process cellular response to cold regulation of microtubule cytoskeleton organization regulation of protein processing cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus postsynaptic modulation of chemical synaptic transmission negative regulation of protein localization to chromatin cAMP/PKA signal transduction positive regulation of protein localization to plasma membrane negative regulation of TORC1 signaling negative regulation of glycolytic process through fructose-6-phosphate protein localization to lipid particle regulation of bicellular tight junction assembly regulation of heart rate renal water homeostasis regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of macroautophagy cytokine-mediated signaling pathway positive regulation of insulin secretion negative regulation of interleukin-2 production high-density lipoprotein particle assembly mitochondrial protein catabolic process positive regulation of calcium-mediated signaling regulation of cardiac muscle contraction cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction vascular endothelial cell response to laminar fluid shear stress regulation of cardiac conduction
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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