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Invitrogen
MARCH5 Polyclonal Antibody
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FIGURE: 1 / 7
MARCH5 Antibody (PA5-25584) in IHC (P)
Immunohistochemistry analysis of MARCH5 in paraffin-embedded Human heart tissue. Samples were incubated with MARCH5 polyclonal antibody (Product # PA5-25584) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody.
Product Details
PA5-25584
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MARCH5
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.50 mg/mL
Amount
200 µg
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, chicken and Xenopus based on sequence homology.
Target Information
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase MARCH5; E3 ubiquitin-protein ligase MARCHF5; MARCH-V; membrane associated ring finger 5; membrane-associated ring finger (C3HC4) 5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V; Mitochondrial ubiquitin ligase; MITOL; RING finger protein 153; RING-type E3 ubiquitin transferase MARCH5; RING-type E3 ubiquitin transferase MARCHF5; unnamed protein product
Gene Aliases: 1810015H18Rik; 2310008I22Rik; 2700055A20Rik; 5730499H23Rik; E130202O05Rik; MARCH-V; MARCH5; MARCHF5; Marchv; MITOL; RNF153
UniProt ID: (Human) Q9NX47, (Mouse) Q3KNM2
Entrez Gene ID: (Human) 54708, (Mouse) 69104
protein binding
zinc ion binding
transferase activity
metal ion binding
GTPase binding
ubiquitin protein ligase activity
ubiquitin-protein transferase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein polyubiquitination
cytoplasmic pattern recognition receptor signaling pathway
protein ubiquitination
negative regulation of viral-induced cytoplasmic pattern recognition receptor signaling pathway
protein K27-linked ubiquitination
NLRP3 inflammasome complex assembly
protein autoubiquitination
protein K63-linked ubiquitination
protein localization to mitochondrion
protein K48-linked ubiquitination
regulation of mitochondrial fission
positive regulation of mitochondrial fission
cGAS/STING signaling pathway
positive regulation of cGAS/STING signaling pathway
positive regulation of NLRP3 inflammasome complex assembly
negative regulation of cell aging
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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