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Invitrogen
TCF4 Polyclonal Antibody
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FIGURE: 1 / 2
TCF4 Antibody (PA5-25659) in WB
Western blot analysis of TCF4 in various lysates. Samples were incubated with TCF4 polyclonal antibody (Product # PA5-25659) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: HepG2 whole cell lysate; Lane 2: K562 whole cell lysate; Lane 3: Mouse cerebellum whole tissue lysate. Predicted band size: 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-25659
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 48-73 amino acids from the N-terminal region of human TCF4
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.4 mg/mL
Amount
160 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Target Information
TCF4 is transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG')-a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Defects in this gene are a cause of Pitt-Hopkins syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: class A helix-loop-helix transcription factor ME2; class B basic helix-loop-helix protein 19; E2 2; immunoglobulin transcription factor 2; immunoglobulin transcription factor-2; ITF 2; MGC149723; MGC149724; MITF-2; SEF 2; SEF2 1; SEF2 1A; SEF2 1B; SL3-3 enhancer factor 2; TCF 4; Transcription factor 4 (Immunoglobulin transcription factor 2) (ITF-2) (MITF-2) (SL3-3 enhancer factor 2) (SEF-2) (Class A helix-loop-helix transcription factor ME2); unnamed protein product
Gene Aliases: 5730422P05Rik; ASP-I2; bHLHb19; CDG2T; E2-2; E2.2; FCD2; FECD3; ITF-2; ITF-2b; ITF2; ME2; MITF-2A; MITF-2B; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; TCF-4; TFE
UniProt ID: (Human) P15884, (Mouse) Q60722
Entrez Gene ID: (Human) 6925, (Mouse) 21413
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
TFIIB-class transcription factor binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
identical protein binding
protein heterodimerization activity
protein dimerization activity
E-box binding
sequence-specific double-stranded DNA binding
double-stranded DNA binding
beta-catenin binding
bHLH transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of gene expression
negative regulation of angiogenesis
endothelial cell activation
positive regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein-DNA complex assembly
regulation of vascular endothelial growth factor signaling pathway
negative regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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