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Invitrogen
IKK epsilon Polyclonal Antibody
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FIGURE: 1 / 4
IKK epsilon Antibody (PA5-29294) in ICC/IF
IKBKE antibody [C2C3], C-term detects IKBKE protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: IKBKE protein stained by IKBKE antibody [C2C3], C-term (Product # PA5-29294) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-29294
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human IKK epsilon. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T.
Predicted reactivity: Bovine (86%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
IKBKE is an activator of nuclear factor-kappa-B. Over expression of IKBKE has been observed in breast cancer cells, and suppression of IKBKE induces cell death. NF-kappa-B is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappa-B is associated with Ikappa-B proteins in the cell cytoplasm, which inhibit NF-kappa-B activity. Ikappa-B is phosphorylated by Ikappa-B kinase (IKK) complex that contains IKK-a, IKK-b, and IKK-gamma. A novel molecule in the IKK complex was recently identified and designated IKK-iota/IKK-i. IKK epsilon is required for the activation of NF-kappa-B by PMA and T cell receptors but not by TNF-a and IL-1. IKK-iota/IKK-i message is expressed in a variety of tissues and is inducible by TNF-a, IL-1, and LPS.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I-kappa-B kinase epsilon; IKK-epsilon; IKK-related kinase epsilon; IKKe; IKK-related kinase; inducible I kappa-B kinase; inducible IkappaB kinase; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; KIAA0151; RP11-534L20.1
Gene Aliases: IKK-E; IKK-i; IKKE; IKKI
UniProt ID: (Human) Q14164
Entrez Gene ID: (Human) 9641
activation of innate immune response
response to stress
immune response
intrinsic apoptotic signaling pathway in response to DNA damage
gene expression
positive regulation of lipid storage
positive regulation of type I interferon production
response to cytokine
response to type I interferon
response to interferon-beta
intracellular signal transduction
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of protein complex assembly
mRNA stabilization
defense response to virus
type I interferon signaling pathway
positive regulation of type I interferon-mediated signaling pathway
interleukin-17-mediated signaling pathway
cellular response to virus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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