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Invitrogen
ATG9B Polyclonal Antibody
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FIGURE: 1 / 2
ATG9B Antibody (PA5-77776) in ICC/IF
Immunofluorescent analysis of ATG9B in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ATG9B polyclonal antibody (Product # PA5-77776) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) ATG9B Antibody D) Merged image. Magnification: 6... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-77776
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide from the N-terminal of Human ATG9B (aa 110-121)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of PA5-77776 was sufficient for detection of ATG9B in 15 µg of Human HeLa Cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 100 kDa.
Target Information
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG9-like 2; ATG9; ATG9 autophagy related 9 homolog B; autophagy 9-like 2 protein; endothelial nitric oxide synthase antisense; nitric oxide synthase 3 antisense; nitric oxide synthase 3-overlapping antisense gene protein; unnamed protein product
Gene Aliases: Apg912; APG9L2; Apgdc2; eONE; Gm574; NOS3AS; SONE
UniProt ID: (Human) Q674R7, (Mouse) Q6EBV9
Entrez Gene ID: (Human) 285973, (Mouse) 213948
autophagosome assembly
mitophagy
autophagy
phospholipid scrambling
protein localization to pre-autophagosomal structure
piecemeal microautophagy of nucleus
bone morphogenesis
reticulophagy
programmed necrotic cell death
establishment of protein localization
chaperone-mediated autophagy
autophagosome organization
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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