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Invitrogen
Phospho-PRKAR2A (Ser96) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-PRKAR2A (Ser96) Antibody (PA5-78122) in WB
Western Blot using Phospho-PRKAR2A (Ser96) Polyclonal Antibody (Product # PA5-78122). Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Phospho-PRKAR2A (Ser96) Polyclonal Antibody (Product # PA5-78122) diluted at 1:3,000. The signal was developed with Trident ECL plus-Enhanced.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78122
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser96 of human PKA R2. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.6 mg/mL
Amount
60 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T
Predicted Reactivity: Chimpanzee (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of AMPK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cAMP-dependent protein kinase regulatory subunit RII alpha; MGC3606; protein kinase A, RII-alpha subunit; protein kinase cAMP-dependent type 2 regulatory subunit alpha; protein kinase type II regulatory subunit (, EC 2.7.1.37); protein kinase, cAMP dependent regulatory, type II alpha; protein kinase, cAMP-dependent, regulatory subunit type II alpha; protein kinase, cAMP-dependent, regulatory, type 2, alpha; unnamed protein product
Gene Aliases: PKR2; PRKAR2
UniProt ID: (Human) P13861, (Rat) P12368
Entrez Gene ID: (Human) 5576, (Rat) 29699
cAMP-dependent protein kinase inhibitor activity
protein binding
cAMP-dependent protein kinase regulator activity
protein domain specific binding
cAMP binding
ubiquitin protein ligase binding
protein kinase A catalytic subunit binding
beta-2 adrenergic receptor binding
small molecule binding
identical protein binding
macromolecular complex binding
adenylate cyclase-activating G-protein coupled receptor signaling pathway
regulation of protein kinase activity
modulation of synaptic transmission
negative regulation of cAMP/PKA signal transduction
regulation of meiotic cell cycle process involved in oocyte maturation
positive regulation of meiotic cell cycle process involved in oocyte maturation
negative regulation of cAMP-dependent protein kinase activity
negative regulation of inflammatory response to antigenic stimulus
renal water homeostasis
chemical synaptic transmission
activation of protein kinase A activity
intracellular signal transduction
cellular response to glucagon stimulus
vascular endothelial cell response to laminar fluid shear stress
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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