Search
Invitrogen
FOXO1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Product Details
PA5-85295
Product Specifications
Species Reactivity
Human,
Rodent,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human FOXO1. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (87%), Rat (92%), Pig (94%).
Positive Control: 293T, A431.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FOXO1 (FKHR, ForKHead Box 01) is a member in a subfamily of the forkhead homeotic gene family of transcription factors. Recent experiments have shown that FOXO1 can act as either a coactivator or a corepressor of nuclear receptor activity that is mediated through the LXXLL motif found in the carboxyl terminal region of the FKHR protein. Association of FOXO1 with PAX3 has been implicated in alveolar rhabdomyosarcoma. Recent studies link the anti-tumor activity of FOXO1, the process of autophagy and myogenic growth and differentiation. FOXO1 is the main target of insulin signaling that regulated the metabolic homeostasis in response to oxidative stress. FOXO1 binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'- TT[G/A]TTTAC-3'. FOXO1 is a regulator of redox balance, osteoblast numbers and controls bone mass. Further, FOXO1 orchestrates the endocrine function of the skeleton in regulating glucose metabolism, and suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, FOXO1 promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1. FOXO1 is an important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOX01A
Gene Aliases: FKH1; FKHR; FOXO1; FOXO1A
UniProt ID: (Human) Q12778
Entrez Gene ID: (Human) 2308
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
nucleic acid binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
beta-catenin binding
chromatin DNA binding
ubiquitin protein ligase binding
sequence-specific DNA binding
protein phosphatase 2A binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
temperature homeostasis
cellular glucose homeostasis
gluconeogenesis
regulation of gluconeogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein acetylation
autophagy
apoptotic process
cellular response to DNA damage stimulus
insulin receptor signaling pathway
cellular response to starvation
gene expression
positive regulation of autophagy
cell differentiation
response to insulin
cellular response to insulin stimulus
negative regulation of stress-activated MAPK cascade
positive regulation of smooth muscle cell apoptotic process
cellular response to oxidative stress
regulation of cell proliferation
glucose homeostasis
positive regulation of apoptotic process
negative regulation of apoptotic process
fat cell differentiation
negative regulation of fat cell differentiation
positive regulation of gluconeogenesis
positive regulation of protein catabolic process
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
canonical Wnt signaling pathway
regulation of transcription initiation from RNA polymerase II promoter
cellular response to cold
response to fatty acid
cellular response to hyperoxia
cellular response to nitric oxide
negative regulation of canonical Wnt signaling pathway
energy homeostasis
neuronal stem cell population maintenance
negative regulation of cardiac muscle hypertrophy in response to stress
regulation of neural precursor cell proliferation
regulation of reactive oxygen species metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support