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Invitrogen
HGF Polyclonal Antibody
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FIGURE: 1 / 3
HGF Antibody (PA5-85536) in WB
Western blot was performed using Anti-HGF Polyclonal Antibody (Product # PA5-85536) and an 83 kDa band corresponding to Hepatocyte growth factor was observed in U-87 MG cells treated with Protein Transport Inhibitor cocktail (500X), 1X for 4 Hrs and absent in NCI-H1299 treated with Protein Transport Inhibitor cocktail (500X), 1X for 4 Hrs along with uncharacterized bands around 30 kDa. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), U-87 MG treated with Protein Transport Inhibitor cocktail (500X), 1X for 4 Hrs (Lane 2), H1299 (Lane... View More
Product Details
PA5-85536
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human HGF (alpha subunit). The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (84%), Rat (85%), Dog (91%), Cat (91%), Rabbit (89%), Bovine (91%).
Positive Control: Huh-7, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin A; Hepatopoietin-A; HepatopoietinA; HGF; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; Scatter factor; scatter factor); SF; unnamed protein product
Gene Aliases: DFNB39; F-TCF; HGF; HGFB; HPTA; SF
UniProt ID: (Human) P14210
Entrez Gene ID: (Human) 3082
mitotic cell cycle
cell morphogenesis
epithelial to mesenchymal transition
liver development
proteolysis
signal transduction
negative regulation of autophagy
skeletal muscle cell proliferation
regulation of anatomical structure morphogenesis
positive regulation of cell migration
negative regulation of interleukin-6 production
positive regulation of interleukin-10 production
cellular response to hepatocyte growth factor stimulus
negative regulation of apoptotic process
positive regulation of MAPK cascade
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
hepatocyte growth factor receptor signaling pathway
animal organ development
epithelial cell proliferation
negative regulation of inflammatory response
positive chemotaxis
regulation of multicellular organismal process
myoblast proliferation
positive regulation of protein kinase B signaling
cell chemotaxis
regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling
negative regulation of release of cytochrome c from mitochondria
regulation of p38MAPK cascade
negative regulation of hydrogen peroxide-mediated programmed cell death
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of DNA biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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