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Invitrogen
CD62E (E-selectin) Polyclonal Antibody
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FIGURE: 1 / 6
CD62E (E-selectin) Antibody (PA5-85877) in ICC/IF
Immunocytochemical analysis of CD62E (E-selectin) in MCF-7 cells using a CD62E (E-selectin) Polyclonal antibody (Product # PA5-85877) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
PA5-85877
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within Human CD62E aa 26-75.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% Glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; unnamed protein product
Gene Aliases: CD62E; ELAM; ELAM1; ESEL; LECAM2; SELE; selectin-e
UniProt ID: (Human) P16581
Entrez Gene ID: (Human) 6401
positive regulation of receptor internalization
leukocyte migration involved in inflammatory response
positive regulation of leukocyte migration
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
phospholipase C-activating G-protein coupled receptor signaling pathway
calcium-mediated signaling
actin filament-based process
response to lipopolysaccharide
response to cytokine
response to tumor necrosis factor
regulation of inflammatory response
leukocyte migration
leukocyte tethering or rolling
response to interleukin-1
cell-cell adhesion via plasma-membrane adhesion molecules
positive regulation of leukocyte tethering or rolling
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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