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Invitrogen
HFE2 Polyclonal Antibody
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FIGURE: 1 / 1
HFE2 Antibody (PA5-92412) in WB
Western blot analysis of HFE2 in extracts of rat heart. Samples were incubated with HFE2 Polyclonal antibody (Product # PA5-92412) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-92412
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-174 of human HFE2.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.13 mg/mL
Amount
113 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: SSPMALGANA TATRKLTIIF KNMQECIDQK VYQAEVDNLP VAFEDGSING GDRPGGSSLS IQTANPGNHV EIQAAYIGTT IIIRQTAGQL SFSIKVAEDV AMAFSAEQDL QLCVGGCPPS QRLSRSERNR RGAITIDTAR RLCKEGLPVE DAYFHSCVFD VLISGDPNFT VAAQAALEDA RAFLPDLEKL HLFPSD
Positive Samples: HeLa, PC-3; Cellular Location: Cell membrane, GPI-anchor, Lipid-anchor
Target Information
HFE2 is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DL M; Fidgetin; haemojuvelin; HEMOCHROMATOSIS; hemochromatosis type 2 (juvenile); hemochromatosis type 2 (juvenile) (human homolog); hemochromatosis type 2 protein; hemochromatosis type 2 protein homolog; hemojuvelin BMP coreceptor; HFE 2; MGC23953; OTTHUMP00000059680; repulsive guidance molecule C; RGM C; RGM domain family member C; transcript variant 1; unnamed protein product
Gene Aliases: 2310035L15Rik; 5230400G09Rik; DL-M; hemojuvelin; HFE2; HFE2A; JH; RGMC
UniProt ID: (Human) Q6ZVN8, (Mouse) Q7TQ32
Entrez Gene ID: (Human) 148738, (Rat) 310681, (Mouse) 69585
negative regulation of transcription from RNA polymerase II promoter
cellular iron ion homeostasis
biological_process
protein autoprocessing
BMP signaling pathway
negative regulation of BMP signaling pathway
activin receptor signaling pathway
positive regulation of transcription from RNA polymerase II promoter
multicellular organismal iron ion homeostasis
cellular response to BMP stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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