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Invitrogen
E6AP Polyclonal Antibody
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FIGURE: 1 / 8
E6AP Antibody (PA5-95918) in ICC/IF
Immunofluorescence analysis of E6AP was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with E6AP Polyclonal Antibody (Product # PA5-95918) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-95918
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.92 mg/mL
Amount
292 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: KLIRLWSKYN ADQIRRMMET FQQLITYKVI SNEFNSRNLV NDDDAIVAAS KCLKMVYYAN VVGGEVDTNH NEEDDEEPIP ESSELTLQEL LGEERRNKKG PRVDPLETEL GVKTLDCRKP LIPFEEFINE PLNEVLEMDK DYTFFKVETE NKFSFMTCPF ILNAVTKNLG LYYDNRIRMY SERRITVLYS LVQGQQLNPY LRLKVRRDHI IDDALVRLEM IAMENPADLK KQLYVEFEGE QGVDEGGVSK EFFQLVVEEI FNPDIGMFTY DESTKLFWFN PSSFETEGQF T; Positive Samples: 293T, SKOV3, BT-474, U-251MG, Jurkat, Mouse brain, Mouse heart, Mouse thymus, Rat brain; Cellular Location: Cytoplasm, Nucleus
Target Information
UBE3A (ubiquitin-protein ligase E3A, AKA E6AP) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A, RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. UBE3A also plays an important role in the regulation of the circadian clock. Defects in UBE3A are a cause of Angelman syndrome (AS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTCL tumor antigen se37-2; E6-AP; E6-AP ubiquitin protein ligase; E6AP ubiquitin-protein ligase; FLJ26981; HECT-type ubiquitin transferase E3A; human papilloma virus E6-associated protein; human papillomavirus E6-associated protein; oncogenic protein-associated protein E6-AP; renal carcinoma antigen NY-REN-54; ubiquitin conjugating enzyme E3A; unnamed protein product
Gene Aliases: 4732496B02; 5830462N02Rik; A130086L21Rik; ANCR; AS; E6-AP; EPVE6AP; HPVE6A; PIX1
UniProt ID: (Human) Q05086, (Mouse) O08759
Entrez Gene ID: (Human) 7337, (Mouse) 22215
protein polyubiquitination
ovarian follicle development
ubiquitin-dependent protein catabolic process
protein ubiquitination
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
positive regulation of protein ubiquitination
cellular response to nutrient levels
response to progesterone
sperm entry
locomotory exploration behavior
regulation of circadian rhythm
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of transcription from RNA polymerase II promoter
regulation of synaptic plasticity
animal organ development
system development
modulation of synaptic transmission
progesterone receptor signaling pathway
protein autoubiquitination
positive regulation of protein kinase B signaling
prostate gland growth
negative regulation of dendritic spine morphogenesis
motor learning
Golgi lumen acidification
protein K48-linked ubiquitination
positive regulation of protein localization to lysosome
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteolysis
brain development
positive regulation of transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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