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CD16 Polyclonal Antibody
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FIGURE: 1 / 1
CD16 Antibody (PA5-96849) in WB
Western blot analysis of CD16 in extracts of various cell lines. Samples were incubated with CD16 Polyclonal antibody (Product # PA5-96849) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-96849
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human FCGR3B (NP_0005613)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.78 mg/mL
Amount
78 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MWQLLLPTAL LLLVSAGMRT EDLPKAVVFL EPQWYSVLEK DSVTLKCQGA YSPEDNSTQW FHNENLISSQ ASSYFIDAAT VNDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKDRKY FHHNSDFHIP KATLKDSGSY FCRGLVGSKN VSSETVNITI TQGLAVSTIS SFSPPGYQVS FCLVMVLLFA VDTGLYFSVK TNI; Positive Samples: Jurkat, Raji, U-937, THP-1, Mouse liver, Mouse spleen, Mouse thymus, Rat spleen; Cellular Location: Cell membrane, GPI-anchor, Lipid-anchor
Target Information
CD16, known as Fc gamma receptor III, exists in two distinct isoforms: Fc gamma RIIIA and Fc gamma RIIIB. These isoforms are encoded by the genes FCGR3A and FCGR3B, respectively, which are located on chromosome 1 and share a high degree of similarity. Fc gamma RIIIA is expressed as a polypeptide-anchored form on monocytes, macrophages, and lymphocytes such as natural killer (NK) cells, but not on T or B cells. This receptor recognizes the Fc portion of immunoglobulin G (IgG) and is involved in the clearance of immune complexes from circulation. Upon binding IgG, Fc gamma RIIIA activates signal transduction pathways that result in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation. Fc gamma RIIIB is expressed on polymorphonuclear neutrophils (PMN) as a glycosylphosphatidylinositol (GPI)-anchored form. It plays a role in similar immune functions, including cellular-mediated cytotoxicity and enhancement of virus infections. Mutations in the FCGR3A gene have been associated with Immunodeficiency 20 and linked to susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD16a antigen; CD32 receptor 2; CDw32; Fc fragment of IgG intermediate affinity IV receptor; Fc fragment of IgG low affinity IIIa receptor (CD16a) isoform FCGR3A_1; Fc fragment of IgG low affinity IIIa receptor (CD16a) isoform FCGR3A_2; Fc fragment of IgG receptor IIIa; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor III-A; Fc gamma receptor III-B; Fc gamma receptor IIIa; Fc gammaRIV; Fc receptor-like 3; Fc-gamma receptor IIIb (CD16); Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma RIII-alpha; fc-gamma RIII-beta; fc-gamma RIIIb; Fc-gamma-R-alpha receptor; Fc-gamma-RIIb; Fc-gamma-RIIc; fc?r; Fcg receptor III; FCG2; FcgammaRIII; FcgammaRIIIA; FcgammaRIIIb; fcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcgRIIIa; FcRII-b; FcRII-c; fcRIII; human neutrophil antigen 1; igG Fc receptor III; igG Fc receptor III-1; igG Fc receptor III-2; IgG Fc receptor III-A; IgG receptor precursor; immunoglobulin G Fc receptor III; integral to membrane; low affinity IgG receptor; low affinity IgG receptor CD16; low affinity IgG receptor precursor; low affinity immunoglobulin gamma Fc region receptor IV; low affinity immunoglobulin gamma receptor III-a Fc fragment; neutrophil-specific antigen NA; RP11-5K23.1; transmembrane receptor FcgammaRIII-X; unnamed protein product
Gene Aliases: 4833442P21Rik; CD16; CD16-2; CD16-I; CD16-II; CD16A; CD16b; FCG3; FcgammaRIV; FCGR3; Fcgr3a; Fcgr4; FcGRIIIA; FcgRIV; FCRIIIA; FCRIIIb; Fcrl3; HNA-1; IGFR3; IMD20
UniProt ID: (Human) P08637, (Human) O75015, (Rat) Q6XPU4, (Mouse) A0A0B4J1G0
Entrez Gene ID: (Human) 2214, (Human) 2215, (Mouse) 14131, (Rat) 304966, (Mouse) 246256
antibody-dependent cellular cytotoxicity
immune response
cell surface receptor signaling pathway
Fc-gamma receptor signaling pathway
dendritic cell antigen processing and presentation
calcium-mediated signaling
natural killer cell activation
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell proliferation
Fc-epsilon receptor signaling pathway
macrophage activation
neutrophil activation
natural killer cell mediated cytotoxicity
natural killer cell degranulation
protein kinase B signaling
positive regulation of bone resorption
cellular response to lipopolysaccharide
Fc receptor-mediated immune complex endocytosis
positive regulation of type IIa hypersensitivity
positive regulation of type III hypersensitivity
positive regulation of type I hypersensitivity
serotonin secretion
phagocytosis, recognition
phagocytosis, engulfment
neutrophil chemotaxis
antigen processing and presentation of exogenous peptide antigen via MHC class I
mast cell activation
positive regulation of phagocytosis
regulation of immune response
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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