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Defined Trypsin Inhibitor - FAQs

Defined Trypsin Inhibitor - FAQs

View additional product information for Defined Trypsin Inhibitor - FAQs (R007100)

9 product FAQs found

Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?

We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Diploid Growth Serum-Reduced Medium (SRM) be supplemented with different types of serum?

Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which cell lines can I culture in Diploid Growth Serum-Reduced Medium (SRM)?

Diploid Growth Serum-Reduced Medium has been evaluated with fibroblast cell lines, including MRC-5, WI-38, IMR-90, BS-2, and CEF cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I use Diploid Growth Serum-Reduced Medium (SRM) supplemented with Diploid Growth Supplement?

Once supplemented, the complete Diploid Growth Serum-Reduced Medium must be used within 4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I freeze or thaw my cells in Diploid Growth Serum-Reduced Medium (SRM)?

Most diploid cell lines can be frozen and thawed in Diploid Growth Serum-Reduced Medium. Consult the Diploid Growth Serum Reduced Medium User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019015_DiploidGrowthSerumReducedMedium_UG.pdf) for additional information.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I adapt my cells to Diploid Growth Serum-Reduced Medium (SRM)?

Cells growing in medium with serum can be transferred directly to Diploid Growth Serum-Reduced Medium without adaptation. Continue to monitor and passage cells for 2-3 passages until consistent growth is achieved. Cultures may benefit from supplementation with 1-2% serum.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is Defined Trypsin Inhibitor a Kunitz-type inhibitor or Bowman-Birk inhibitor?

Defined Trypsin Inhibitor (Cat. No. R007100) is the larger trypsin inhibitor, therefore it is a Kunitz-type inhibitor

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.