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Invitrogen
Human HGFR (c-MET) His-tag Recombinant Protein
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Product Details
RP-88029
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human c-Met, amino acids Met1-Thr932 (Accession # NP_000236.2) with a C-terminal His-tag
Tag
His-tag
Molecular weight
103 kDa
Class
Recombinant
Type
Protein
Purity
≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized Cynomolgus HGF at 2 µg/mL (100 µL/well) can bind Human c-MET His, the EC50 of Human c-MET His is 16-48 ng/mL.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.25 mg/mL once reconstituted
Amount
100 µg
Purification
purified
Storage buffer
20mM tris, pH 8.5, with 5% mannitol, 150mM NaCl, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
Reconstituted with sterile deionized water to 0.25 mg/mL.
Concentration is measured by UV.
Target Information
MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cMet; EC 2.7.10.1; HGF receptor; HGF-SF receptor; HGF/SF receptor; met oncogene; MET proto-oncogene protein; oncogene MET; proto-oncogene c-Met; sc MET; scatter factor receptor; SF receptor; soluble c met; tyrosine kinase; hepatocyte growth factor receptor; HGFR; tyrosine-protein kinase Met; unnamed protein product
Gene Aliases: AUTS9; c-Met; DA11; DFNB97; HGFR; RCCP2
UniProt ID: (Human) P08581
Entrez Gene ID: (Human) 4233
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