DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples
DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples
Citations & References (10)
Invitrogen™

DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples

DNAzol™試薬は、動物、植物、酵母、細菌由来の固体および液体サンプルからゲノムDNAを分離するための完全ですぐに使える有機試薬です。DNAzol™試薬の手順は、通常10～30分で完了し、DNA回収率は70～100%です。DNAzol™ 試薬は：•高い汎用性—さまざまなサンプルに対応可能です•詳細を見る
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製品番号（カタログ番号）数量
10503027100 mL
製品番号（カタログ番号） 10503027
価格（JPY）
45,200
Each
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数量:
100 mL
DNAzol™試薬は、動物、植物、酵母、細菌由来の固体および液体サンプルからゲノムDNAを分離するための完全ですぐに使える有機試薬です。DNAzol™試薬の手順は、通常10～30分で完了し、DNA回収率は70～100%です。DNAzol™ 試薬は：

•高い汎用性—さまざまなサンプルに対応可能です
• 効率的—ゲノムDNAの迅速な分離と高い回収率を実現

さまざまなサンプルタイプからゲノムDNAを効率的に分離できます
DNAzol™試薬の手順では、細胞溶解液からDNAを選択的に沈殿させることができる新規のグアニジン-界面活性剤溶解液を使用しています。1 mLのDNAzol™試薬を使用して、1～3 x 107細胞、0.1 mLの全血、または 25～50 mgの組織からゲノムDNAを分離できます。

ゲノムDNAの迅速な分離と高い回収率
™ DNAzol試薬は、そのDNA分離プロトコルにおいて、信頼性、高い効率、およびシンプルを兼ね備えています。分離中、生体サンプルはDNAzol™試薬で溶解（またはホモジナイズ）され、そのゲノムDNAがエタノールで溶解液から沈殿させられます。エタノール洗浄後、DNAは水または8 mm NaOHのいずれかで可溶化されることができます。全手順は、10～30分で完了し、DNAの回収率は70～100%です。

分離されたDNAは多くのダウンストリームアプリケーションに使用できます。
DNAzol™法で抽出したゲノムDNAは、サザンブロッティング、クローニング、PCR、制限酵素消化、ドットブロットハイブリダイゼーションなど、さまざまな用途に適しています。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
溶出量Varies
最終産物タイプゲノムDNA
使用対象（アプリケーション）PCR、サザンブロッティング、シーケンシング、クローニング
高スループット適合性ハイスループット非対応（手動）
数量100 mL
サンプルタイプ細胞、組織, Cells, Plant, Tissue, Yeast
出荷条件室温
出発物質量Bacteria: ≤107 cells or 0.1 mL
Cells: ≤107
Plant: ≤50 mg
Tissue: ≤50 mg
Yeast: ≤107 cells or 0.1 mL
検査時間30分
Isolation Technology有機物抽出
Unit SizeEach
組成および保存条件
内容：ボトル（100 mL）1本
15℃～30℃で保管してください。

よくあるご質問（FAQ）

I see two phases after addition of ethanol during DNA isolation using DNAzol Reagent. What should I do?

It is possible to see two phases after addition of ethanol if the amount of DNAzol Reagent was too low. Add more DNAzol Reagent and continue.

I've isolated my DNA using the DNAzol Reagent, but cannot cut it with restriction enzymes. What could be causing this?

If the cells or tissue were washed with phosphate buffer solutions prior to DNA isolation, the phosphate may have been carried over and be inhibiting restriction enzymes. We recommend adding DNAzol Reagent to the DNA solution and reprecipitating with 0.5 volumes of 95% EtOH. Wash twice with 95%, dry briefly, and resuspend in 8 mM NaOH.

The DNA I've isolated using the DNAzol Reagent is hard to resuspend. Do you have recommendations for fixing this?

You can try incubating samples resuspended in 8 mM NaOH at 37 degrees C overnight to resuspend the DNA. You can also try incubating at 45 degrees C for 15 minutes.

My DNAzol Reagent turned a dark murky green from its original light green. Is it still okay to use?

Yes, this happens due to the dye in the reagent, and seems to be dependent on the volume of the stored reagent. The color change does not affect its performance.

Will DNAzol Reagent isolate only genomic DNA or will plasmid DNA also be isolated? How about mitochondrial DNA?

The DNA isolated is actually total DNA, so plasmid DNA will be isolated along with genomic DNA. The mitochondrial genome is similar to a plasmid and can be isolated using DNAzol Reagent. The 1 minute room temperature incubation in ethanol before centrifugation should be extended to 5-10 minutes for maximum recovery.

View all DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples FAQs

引用および参考文献 (10)

引用および参考文献
Abstract
p21Cip-1/SDI-1/WAF-1 gene is involved in chondrogenic differentiation of ATDC5 cells in vitro.
Authors: Negishi Y; Ui N; Nakajima M; Kawashima K; Maruyama K; Takizawa T; Endo H;
Journal:J Biol Chem
PubMed ID:11406616
'Development of skeletal cartilage is characterized with coupling growth arrest and cell differentiation. Here, to understand the cyclin-dependent kinase inhibitors involved in the progression of chondrogenic differentiation, we examined changes in the expression levels of cyclin-dependent kinase inhibitor members using mouse ATDC5 prechondrocytes as a widely used in vitro model ... More
A new type of high affinity folic acid transporter in the protozoan parasite Leishmania and deletion of its gene in methotrexate-resistant cells.
Authors:Richard D, Kundig C, Ouellette M.
Journal:J Biol Chem
PubMed ID:12023977
'The protozoan parasite Leishmania is a folate auxotroph and thus depends on the uptake of folate from the environment to meet its folate requirement. We show here that Leishmania contains several putative pteridine transporter genes. Some of these genes are deleted in methotrexate-resistant Leishmania cells where there is no measurable ... More
Isolation of high-molecular-weight genomic DNA from intact biohazardous mammalian tissues.
Authors:Liska V; Ruprecht R M;
Journal:Biotechniques
PubMed ID:9894593
n/a
DNA extraction from fixed cytogenetic cell suspensions.
Authors:Amorim MR, Vargas FR, Llerena JC, Pombo-de-Oliveira MS
Journal:Genet Mol Res
PubMed ID:17985302
We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment ... More
Rapid and efficient extraction of genomic DNA from different phytopathogenic fungi using DNAzol reagent.
Authors:Guo JR, Schnieder F, Abd-Elsalam KA, Verreet JA
Journal:Biotechnol Lett
PubMed ID:15685411
A modified procedure using the commercial DNAzol reagent was successfully applied to extract genomic DNA from 25 fungal species. The DNA yield varied from 306 to 1,927 microg g(-1) dry mycelia and the A(260)/A(280) ratio from 1.59 to 1.93. Compared with the method of J.L. Cenis (Nucleic Acids Res. 1992, ... More
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