RNaseOUT™ Recombinant Ribonuclease Inhibitor

Citations & References (6)

Invitrogen™

RNaseOUT™ Recombinant Ribonuclease Inhibitor

製品番号（カタログ番号）	数量
10777019	5000 Units

製品番号（カタログ番号） 10777019

価格（JPY）

34,600

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5000 Units

一括またはカスタム形式をリクエストする

RNaseOUT™組換え型リボヌクレアーゼ阻害剤は、RNase Aなどの膵臓リボヌクレアーゼの強力な非競合阻害剤で、さまざまなアプリケーションにおけるRNAの分解を防止するために使用されます。RNaseOUT™組み換え型リボヌクレアーゼ阻害剤は、分子量約52 kDaの酸性タンパク質です。RNaseOUT™は、RNase A、RNase B、およびRNase Cを阻害します。

アプリケーション
cDNAの合成、RT-PCR、in vitroでの転写および翻訳

ソース
クローン化されたブタ遺伝子を発現する大腸菌からアフィニティークロマトグラフィーで精製

性能および品質検査
SDS-PAGE純度、制限エンドヌクレアーゼアッセイ、タンパク質濃度、比活性度、RT-PCRで評価した性能

ユニットの定義
1ユニットで、シチジン2',3'環状一リン酸（cCMP）を基質とする5 ngのRNase Aを50％阻害

ユニットの反応条件
100 mMトリス-アセテート（pH6.5）、1 mM EDTA、0.2 mM cCMP、2 µgのRNase Aを1 mLに入れ、25℃で0～10分放置

研究用にのみ使用できます。診断用には使用いただけません。

濃度100 mM

製品ラインRnaseOUT

数量5000 Units

Unit SizeEach

組成および保存条件

-20℃で保存製品を頻繁に温度変化にさらさないでください。RnaseOUT™リボヌクレアーゼ阻害剤は、活性を維持するために1 mmのDTTを必要とします。

製品マニュアルに特に記載がない限り、本製品の保証期間は、購入日から6ヶ月間です。

よくあるご質問（FAQ）

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

What does RNaseOUT Recombinant RNase Inhibitor do?

The inhibitor acts as a safeguard against degradation of target RNA due to ribonuclease contamination of the RNA preparation.

What is the concentration of RNaseOUT Recombinant Ribonuclease Inhibitor in Units/microL?

RNaseOUT Recombinant Ribonuclease Inhibitor is provided at a concentration of 40 U/µL.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the temperature limitation of RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)?

RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019) unit reaction conditions are defined at 25 degrees C. The upper temperature limit for full functionality is 40 degrees C. The enzyme half-life is decreased as temperatures increase above 40 degrees C. There is some residual activity up to 50-55 degrees C but heating at 65 degrees C will inactivate the enzyme. As RNaseOUT can be used in various applications like cDNA synthesis, RT-PCR, and in vitro transcription, the recommendation is to follow the temperature settings required for the respective method.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I am having trouble performing PCR amplification after DNA isolation with RNAlater-treated samples. Does RNAlater have an impact on downstream PCR applications?

RNAlater should not impact downstream PCR amplification as long as the sample has been properly cleaned before proceeding with DNA isolation, as stated in the RNAlater Stabilization Solution manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/7020M.pdf) on page 7.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

View all RNaseOUT™ Recombinant Ribonuclease Inhibitor FAQs

引用および参考文献 (6)

引用および参考文献

Abstract

The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection.

Authors: Feng Yanan; Vickers Timothy A; Cohen Stanley N;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12417756

'RNase E, a multifunctional endoribonuclease of Escherichia coli, attacks substrates at highly specific sites. By using synthetic oligoribonucleotides containing repeats of identical target sequences protected from cleavage by 2''-O-methylated nucleotide substitutions at specific positions, we investigated how RNase E identifies its cleavage sites. We found that the RNase E catalytic ... More

Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family.

Authors: Gao Lin; Cueto Maria A; Asselbergs Fred; Atadja Peter;

Journal:J Biol Chem

PubMed ID:11948178

'We have cloned and characterized a human cDNA that belongs to the histone deacetylase family, which we designate as HDAC11. The predicted HDAC11 amino acid sequence reveals an open reading frame of 347 residues with a corresponding molecular mass of 39 kDa. Sequence analyses of the putative HDAC11 protein indicate ... More

Development of reverse transcription (RT)-PCR and real-time RT-PCR assays for rapid detection and quantification of viable yeasts and molds contaminating yogurts and pasteurized food products.

Authors:Bleve G, Rizzotti L, Dellaglio F, Torriani S,

Journal:Appl Environ Microbiol

PubMed ID:12839789

Reverse transcriptase PCR (RT-PCR) and real-time RT-PCR assays have been used to detect and quantify actin mRNA from yeasts and molds. Universal primers were designed based on the available fungal actin sequences, and by RT-PCR they amplified a specific 353-bp fragment from fungal species involved in food spoilage. From experiments ... More

Regulation of H-ras splice variant expression by cross talk between the p53 and nonsense-mediated mRNA decay pathways.

Authors:Barbier J, Dutertre M, Bittencourt D, Sanchez G, Gratadou L, de la Grange P, Auboeuf D,

Journal:Mol Cell Biol

PubMed ID:17709397

When cells are exposed to a genotoxic stress, a DNA surveillance pathway that involves p53 is activated, allowing DNA repair. Eukaryotic cells have also evolved a mechanism called mRNA surveillance that controls the quality of mRNAs. Indeed, mutant mRNAs carrying premature translation termination codons (PTCs) are selectively degraded by the ... More

Biochemistry of mitochondrial nitric-oxide synthase.

Authors:Elfering SL, Sarkela TM, Giulivi C

Journal:J Biol Chem

PubMed ID:12154090

We reported that the generation of nitric oxide by mitochondria is catalyzed by a constitutive, mitochondrial nitric-oxide synthase (mtNOS). Given that this production may establish the basis for a novel regulatory pathway of energy metabolism, oxygen consumption, and oxygen free radical production, it becomes imperative to identify unequivocally and characterize ... More

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