'Progenitor and pluripotent cell types offer promise as regenerative therapies but transfecting these sensitive cells has proven difficult. Herein, a series of linear trehalose-oligoethyleneamine "click" copolymers were synthesized and examined for their ability to deliver plasmid DNA (pDNA) to two progenitor cell types, human dermal fibroblasts (HDFn) and rat mesenchymal ... More
Comparison of conventional PCR, quantitative PCR, bacteriological culture and the Warthin Starry technique to detect Leptospira spp. in kidney and liver samples from naturally infected sheep from Brazil.
Authors:Fornazari F, da Silva RC, Richini-Pereira VB, Beserra HE, Luvizotto MC, Langoni H
Journal:J Microbiol Methods
PubMed ID:22713608
'Leptospirosis is an infectious disease of worldwide importance. The development of diagnostic techniques allows sick animals to be identified, reservoirs to be eliminated and the disease prevented and controlled. The present study aimed to compare different techniques for diagnosing leptospirosis in sheep. Samples of kidney, liver and blood were collected ... More
Periodontopathogens around the surface of mini-implants removed from orthodontic patients.
Authors:Tortamano A, Dominguez GC, Haddad AC, Nunes FD, Nacao M, Morea C
Journal:Angle Orthod
PubMed ID:22839769
To verify if mini-implant mobility is affected by the presence of periodontopathogens, frequently associated with peri-implantitis.
Microarray-CGH for the assessment of aneuploidy in human polar bodies and oocytes.
Authors:Jaroudi S, Wells D
Journal:Methods Mol Biol
PubMed ID:23138959
The cytogenetic analysis of single cells, such as oocytes and polar bodies, is extremely challenging. The main problem is low probability of obtaining a metaphase preparation in which all of the chromosomes are sufficiently well spread to permit accurate analysis (no overlapping chromosomes, no chromosomes lost). As a result, a ... More
Cloning of cytokine 3' untranslated regions and posttranscriptional assessment using cell-based GFP assay.
Authors:Al-Haj L, Khabar KS
Journal:Methods Mol Biol
PubMed ID:22131027
Cytokine biosynthesis is tightly regulated by a number of processes, including gene expression control. Posttranscriptional control of cytokine gene expression offers a fine-tuning mechanism that contributes not only to transient biosynthesis of cytokines, but also helps in rapid and early initiation of the cytokine response. Deregulation of cytokine biosynthesis has ... More