Grace's Insect Medium, supplemented
Grace's Insect Medium, supplemented
Gibco™

Grace's Insect Medium, supplemented

グレース昆虫細胞培養培地(サプリメント含有)は、Australian Emperor Gum Moth(Antherea eucalypti)由来細胞の増殖用です詳細を見る
製品番号(カタログ番号)数量
11605094500 mL
1160510210 x 500 mL
製品番号(カタログ番号) 11605094
価格(JPY)
6,400
Each
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数量:
500 mL
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グレース昆虫細胞培養培地(サプリメント含有)は、Australian Emperor Gum Moth(Antherea eucalypti)由来細胞の増殖用です。グレース昆虫細胞培養培地(サプリメント含有)は、Spodepta Frugiperda細胞(Sf9およびSf21)の増殖に広く使用されています。

Sf9およびSf21細胞は、バキュロウイルス発現ベクターシステム(Baculovirus Expression Vector System: BEVS)を使用して組換えタンパク質を発現するために日常的に使用されています。幅広い昆虫細胞培養用途向けに、さまざまなGibco™ 培地をご用意しています。

組成
当社のグレース昆虫細胞培養培地(サプリメント含有)には、ラクトアルブミン加水分解物、イーストレート、L-グルタミン酸、重炭酸ナトリウムが含まれています。詳細なfor組成をご利用いただけます。グレース昆虫細胞培養培地(サプリメント含有)には、サプリメント不含培地には一般的に含まれないラクトアルブミン加水分解物とイーストレートが含まれています。

2施設cGMP準拠の製造および品質管理システム
Gibco™ グレース昆虫細胞培養培地(サプリメント含有)は、ニューヨーク州グランドアイランドにあるcGMP準拠施設で製造されています。この施設は医療機器製造業者としてFDAに登録されており、ISO 13485の認証を受けています。また、サプライチェーン継続性のため、当社のスコットランド施設で製造した同一製品(11605-045)も提供しています。この施設は医療機器製造業者としてFDAに登録されており、ISO 13485規格の認証を受けています。

グレース昆虫細胞培養培地(サプリメント含有)には、ラクトアルブミン加水分解物およびイーストレートが含まれています。通常は、使用前に10%ウシ胎児血清(FBS)を添加します。グレース昆虫細胞培養培地(サプリメント含有)は、生理学的pHの維持にCO2雰囲気を必要とします。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
細胞株Sf21, Sf9
細胞タイプInsect Cell
無機塩類Calcium, Magnesium
製品ラインGibco
製品タイプGrace's Insect Medium
数量500 mL
出荷条件Room Temperature
形状Liquid
添加剤ありGlutamine, Yeastolate, Lactalbumin Hydrolysate
添加剤なしNo Insect Hemolymph
Unit SizeEach
組成および保存条件
Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

よくあるご質問(FAQ)

For how long can I store Grace's Insect Medium, unsupplemented at room temperature?

We ship this product under “ambient” conditions throughout the year. We have performed shipping studies that shown components to be stable for at least 7 days at ambient temperatures. The important factor is to ensure that the media is not exposed to light at the warmer temperatures as there are vitamins and amino acids that will breakdown with light exposure.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?

Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the “base” growth medium. For the “complete” growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This “complete” growth medium is also referred to as TNM-FH.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用および参考文献 (9)

引用および参考文献
Abstract
Bombyxin is a growth factor for wing imaginal disks in Lepidoptera.
Authors: Nijhout H Frederik; Grunert Laura W;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12429853
'The mechanisms that control the growth rate of internal tissues during postembryonic development are poorly understood. In insects, the growth rate of imaginal disks varies with nutrition and keeps pace with variation in somatic growth. We describe here a mechanism by which the growth of wing imaginal disks is controlled. ... More
Kinesin Superfamily Motor Protein KIF17 and mLin-10 in NMDA Receptor-Containing Vesicle Transport
Authors:Mitsutoshi Setou, Terunaga Nakagawa, Dae-Hyun Seog, Nobutaka Hirokawa *
Journal:Science
PubMed ID:10846156
'Experiments with vesicles containing N-methyl-D-aspartate (NMDA)receptor 2B (NR2B subunit) show that they are transported alongmicrotubules by KIF17, a neuron-specific molecular motor in neuronaldendrites. Selective transport is accomplished by direct interaction of theKIF17 tail with a PDZ domain of mLin-10 (Mint1/X11), which is aconstituent of a large protein complex including mLin-2 ... More
Interaction of nucleotides with membrane-associated cystic fibrosis transmembrane conductance regulator.
Authors:Travis SM, Carson MR, Ries DR, Welsh MJ
Journal:J Biol Chem
PubMed ID:7687995
'Cystic fibrosis transmembrane conductance regulator (CFTR) is a Cl- channel that is regulated by cytosolic nucleotides and by cAMP- dependent phosphorylation. In excised membrane patches, CFTR Cl- channel activity requires hydrolyzable nucleotides and Mg2+, and is inhibited by ADP. We examined the interactions between CFTR and nucleotides using 8-azidoadenosine 5''-triphosphate ... More
Purification, reconstitution, and steady-state kinetics of the trans-membrane 17 beta-hydroxysteroid dehydrogenase 2.
Authors: Lu Ming-Liang; Huang Yi-Wei; Lin Sheng-Xiang;
Journal:J Biol Chem
PubMed ID:11940569
'Human membrane 17 beta-hydroxysteroid dehydrogenase 2 is an enzyme essential in the conversion of the highly active 17beta-hydroxysteroids into their inactive keto forms in a variety of tissues. 17 beta-hydroxysteroid dehydrogenase 2 with 6 consecutive histidines at its N terminus was expressed in Sf9 insect cells. This recombinant protein retained ... More
Estimation of baculovirus titer by beta-galactosidase activity assay of virus preparations.
Authors:Yahata T, Andriole S, Isselbacher KJ, Shioda T
Journal:Biotechniques
PubMed ID:10948416
There is no abstract available. The title describes the paper.