Antibiotic-Antimycotic (100X)
Antibiotic-Antimycotic (100X)
Citations & References (4)
Gibco™

Antibiotic-Antimycotic (100X)

Gibco™抗生物質-抗真菌剤は、細菌汚染および真菌汚染を防止するために使用されます。この溶液には、10,000ユニット／mLのペニシリン、10,000 µg/mLのストレプトマイシン、および25 µg/mLのGibcoアンフォテリシンBが含まれています。抗生物質のペニシリンとストレプトマイシンは、グラム陽性菌とグラム陰性菌に対する効果的な組み合わせ作用により詳細を見る
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製品番号（カタログ番号）数量
1524011220 x 100 mL
1524009620 mL
15240062100 mL
製品番号（カタログ番号） 15240112
価格（JPY）
166,700
Each
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数量:
20 x 100 mL
Gibco™抗生物質-抗真菌剤は、細菌汚染および真菌汚染を防止するために使用されます。この溶液には、10,000ユニット／mLのペニシリン、10,000 µg/mLのストレプトマイシン、および25 µg/mLのGibcoアンフォテリシンBが含まれています。抗生物質のペニシリンとストレプトマイシンは、グラム陽性菌とグラム陰性菌に対する効果的な組み合わせ作用により、細胞培養の細菌汚染を防ぎます。アムホテリシンBは、多細胞性菌類および酵母の阻害により、細胞培養の真菌汚染を防止します。

ペニシリンは菌類青カビから独自に精製され、細菌細胞壁の代謝回転を直接的に妨害することで作用し、細胞壁をさらに変化させる酵素の放出を誘発することによって間接的に作用します。ストレプトマイシンは、ストレプトマイセスグリセウスから精製されました。細菌リボソームの30Sサブユニットに結合することで作用し、タンパク質合成を阻害し、感受性細菌を死滅させます。アムホテリシンBは、真菌性血漿膜透過性を高めることで真菌類や酵母の成長を防止する抗真菌剤です。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
濃度100 X
培養タイプMammalian Cell Culture
数量20 x 100 mL
品質保持期間12 Months
形状Liquid
製品タイプAntibiotic-Antimycotic
無菌性Sterile-filtered
Sterilization MethodSterile-filtered
Unit SizeEach
組成および保存条件
Storage conditions: -5°C to -20°C
Shipping conditions: Dry ice
Shelf life: 12 months from date of manufacture

よくあるご質問（FAQ）

Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?

Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the solvent used in Antibiotic-Antimycotic (100X)?

The components are in a 0.85% saline solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the components of Antibiotic-Antimycotic (100X)?

This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition for Antibiotic-Antimycotic (100X)?

The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Should I directly add 5 mL of Antibiotic-Antimycotic (100X) to 500 mL cell culture medium or should I dilute the Antibiotic-Antimycotic (100X) 100 times and then add 5 ml of the diluted Antibiotic-Antimycotic to 500 ml of cell culture medium?

The accurate way would be to dilute 5 mL of Antibiotic-Antimycotic (100X) into 495 mL of medium. Please note that the volume of medium in a 500 mL medium bottle is not exactly 500 mL. It could range from 500.5 - 510.5 mL.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Antibiotic-Antimycotic (100X) FAQs

引用および参考文献 (4)

引用および参考文献
Abstract
Common variation at 6q16 within HACE1 and LIN28B influences susceptibility to neuroblastoma.
Authors:Diskin SJ, Capasso M, Schnepp RW, Cole KA, Attiyeh EF, Hou C, Diamond M, Carpenter EL, Winter C, Lee H, Jagannathan J, Latorre V, Iolascon A, Hakonarson H, Devoto M, Maris JM,
Journal:Nat Genet
PubMed ID:22941191
Neuroblastoma is a cancer of the sympathetic nervous system that accounts for approximately 10% of all pediatric oncology deaths. Here, we report a genome-wide association study of 2,817 neuroblastoma cases and 7,473 controls. We identified two new associations at 6q16, the first within HACE1 (rs4336470; combined P=2.7×10(-11); odds ratio 1.26, ... More
Wnt-induced activation of glucose metabolism mediates the in vivo neuroprotective roles of Wnt signaling in Alzheimer disease.
Authors:Cisternas P, Zolezzi JM, Martinez M, Torres VI, Wong GW, Inestrosa NC
Journal:J Neurochem
PubMed ID:30300917
'Dysregulated Wnt signaling is linked to major neurodegenerative diseases, including Alzheimer disease (AD). In mouse models of AD, activation of the canonical Wnt signaling pathway improves learning/memory, but the mechanism for this remains unclear. The decline in brain function in AD patients correlates with reduced glucose utilization by neurons. Here, ... More
Human antigen R protein modulates vascular endothelial growth factor expression in human corneal epithelial cells under hypoxia.
Authors:Hung KF, Sun YC, Liou HM, Hu FR
Journal:J Formos Med Assoc
PubMed ID:31262614
'Corneal avascularity is critical for corneal transparency; therefore, a tailored process has been presumed to minimize corneal neovascularization (NV). In most cell types, the transcription of vascular endothelial growth factor (VEGF) is up-regulated, and the stability of VEGF mRNA is sustained by human antigen R (HuR) during hypoxia; however, whether ... More
Increased O-GlcNAcylation of c-Myc Promotes Pre-B Cell Proliferation.
Authors:Lee DH, Kwon NE, Lee WJ, Lee MS, Kim DJ, Kim JH, Park SK
Journal:Cells
PubMed ID:31936366
O-linked ß-N-acetylglucosamine (O-GlcNAc) modification regulates the activity of hundreds of nucleocytoplasmic proteins involved in a wide variety of cellular processes, such as gene expression, signaling, and cell growth; however, the mechanism underlying the regulation of B cell development and function by O-GlcNAcylation remains largely unknown. Here, we demonstrate that changes ... More